Methanol extraction fraction from Citrus Sudachi peel exerts lipid reducing effects in cultured cells
| Autor: | Yoshiki Kashiwada, Tomomi Yamaoka, Naonobu Tanaka, Toshiaki Tamaki, Wenting Xu, Haruna Aihara, Koichiro Tsuchiya, Yasumasa Ikeda, Yuki Tsuchihashi, Licht Miyamoto |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Citrus 030209 endocrinology & metabolism AMP-Activated Protein Kinases Models Biological Ectopic fat General Biochemistry Genetics and Molecular Biology Cell Line Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Insulin resistance Sirtuin 1 Diabetes mellitus medicine Sudachi Animals Humans PPAR alpha Food science Triglycerides Hypolipidemic Agents chemistry.chemical_classification Triglyceride biology Plant Extracts Methanol Fatty acid AMPK Lipid metabolism General Medicine Lipid Metabolism medicine.disease biology.organism_classification Citrus Sudachi 030104 developmental biology chemistry 5’AMP-activated protein kinase Intracellular Phytotherapy Signal Transduction |
| Zdroj: | The Journal of Medical Investigation. 65:225-230 |
| ISSN: | 1349-6867 1343-1420 |
| DOI: | 10.2152/jmi.65.225 |
| Popis: | Ectopic fat accumulation is associated with insulin resistance and type 2 diabetes mellitus. Citrus sudachi is an evergreen tree that is found mainly in Tokushima Prefecture in Japan. Previously, it was demonstrated that Citrus sudachi could inhibit the rising trend of blood glucose and fatty acid in human subjects. In the current study, we illustrated the function of methanol extracts from sudachi peel and investigated the mechanism of this effect. We got the five kinds of methanol extracts by using diaion HP-20, and those were named by hydrophobicity from M-F1 to M-F5. Among the 5 kinds of sudachi methanol extracts, only M-F4 significantly decreased the intracellular triglyceride of C2C12 cells. It augmented the AMPK activity and increased the transcription of PPARα and its downstream targets CPT-1b and UCP2. In conclusion, M-F4 improved the lipid metabolism possibly through AMPK, PPARα and their downstream targets like CPT-1b and UCP2. Furthermore, this extract may be useful for preventing obesity and diabetes related diseases. J. Med. Invest. 65:225-230, August, 2018. |
| Databáze: | OpenAIRE |
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