Tracer studies of the interconversion of R- and S-methylmalonic semialdehydes in man
| Autor: | R J Pollitt, N J Manning |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Male
Stereochemistry Sodium Hydroxybutyrates chemistry.chemical_element Dehydrogenase Urine Biochemistry Excretion chemistry.chemical_compound Isobutyrates Isomerism Malondialdehyde Labelling Humans Molecular Biology Chemistry Catabolism Cell Biology Metabolism Chromatography Ion Exchange Deuterium Malonates Thymine Butyrates Research Article |
| Zdroj: | Biochemical Journal. 231:481-484 |
| ISSN: | 1470-8728 0264-6021 |
| DOI: | 10.1042/bj2310481 |
| Popis: | Two human subjects were given separate oral doses of sodium [2H6]isobutyrate and [methyl-2H3]thymine and the labelling patterns of urinary metabolites were determined. Ingestion of deuterated isobutyrate resulted in the excretion of 2H5-labelled S-3-hydroxyisobutyric acid, formed on the direct catabolic pathway, and of S- and R-[2H4]-3-hydroxyisobutyric acids, formed by the reduction of S- and R-methylmalonic semialdehydes respectively. Only the R-enantiomer of urinary 3-hydroxyisobutyric acid was labelled by thymine. This labelling pattern indicates a flow from S- to R-methylmalonic semialdehyde, suggesting that the R-enantiomer is the substrate of methylmalonic semialdehyde dehydrogenase. |
| Databáze: | OpenAIRE |
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