Expression of Platelet-Derived Growth Factor β Receptor in Chondrogenesis of Perichondrial Transplants
| Autor: | Bertil Widenfalk, Anders Ljung, Monica Nistér, Valdemar Skoog, Lennart Ohlsén, Zhi-Ping Ren |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
medicine.medical_specialty
Platelet-derived growth factor medicine.medical_treatment Ribs chemistry.chemical_compound Internal medicine Gene expression medicine Animals Perichondrium Knee RNA Messenger In Situ Hybridization Platelet-Derived Growth Factor business.industry Growth factor Graft Survival General Medicine Chondrogenesis Immunohistochemistry Transplantation Cartilage surgical procedures operative Endocrinology Cytokine chemistry Rabbits business |
| Zdroj: | Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery. 29:289-295 |
| ISSN: | 0284-4311 |
| DOI: | 10.3109/02844319509008962 |
| Popis: | An experimental study was done in two rabbits to establish whether the early stages of cartilage generation involves stimulation via the platelet-derived growth factor beta receptor (PDGFR-beta). Rib perichondrium was dissected off and transplanted to the knee joint. The perichondrial grafts were removed for microscopic investigation at day 0 and day 6 and kept frozen until analysed by immunohistochemistry and in situ hybridisation for the presence of PDGF beta receptor protein and mRNA, respectively. Samples from day 0 showed sparse cells with a positive immunohistochemical reaction. In samples from day 6 there were signs of perichondrial proliferation and chondroid maturation and a more widespread immunohistochemical staining reaction could be seen mainly on proliferating perichondrial cells, but also on some chondrocytes. In situ hybridisation confirmed the expression of PDGF beta receptor mRNA in the same areas of serial sections. These findings may indicate that PDGF beta receptor activity is part of the early response after autotransplantation of perichondrium. |
| Databáze: | OpenAIRE |
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