Identification of proteins associated with the formation of oral biofilms
| Autor: | Margarita F. Requena-Mendizabal, Reyma Evelyn Bacilio Amaranto, Pablo Alejandro Millones-Gómez, Dora Maurtua Torres, Rubén Calla-Poma, Roger Damaso Calla-Poma, María Isabel Alvino-Vales |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
gene amplification
polymerase chain reaction acetone tooth plaque protein fingerprinting bacterial growth biofilm Western blotting Peptide mass fingerprinting pyroxylin Polyacrylamide gel electrophoresis Glyceraldehyde 3-phosphate dehydrogenase biology Chemistry alcohol ultrasound Streptococcus gordonii RK1-715 Genomics saliva substitute adhesion trypsin real time polymerase chain reaction glyceraldehyde 3 phosphate dehydrogenase (NADP) diaminobenzidine oligonucleotide Dental Plaque bacterium culture hydrogen peroxide glycerol Dental plaque colony formation Article Microbiology stomatognathic system medicine genomics carboxymethylcellulose cytoplasm protein General Dentistry protein expression centrifugation phosphate buffered saline nonhuman Fusobacterium nucleatum Biofilm biochemical phenomena metabolism and nutrition medicine.disease biology.organism_classification bacterial strain stomatognathic diseases Fusobacterium protein analysis Dentistry electrophoresis Pediatrics Perinatology and Child Health biology.protein heat shock protein 40 polyacrylamide gel electrophoresis |
| Zdroj: | Pesquisa Brasileira em Odontopediatria e Clínica Integrada, Vol 21 (2021) Universidad Privada San Juan Bautista Repositorio Institucional UPSJB UPSJB-Institucional instacron:UPSJB Pesquisa Brasileira em Odontopediatria e Clínica Integrada, Volume: 21, Article number: e0128, Published: 30 JUN 2021 |
| Popis: | Objective: To identify proteins associated with the formation of Streptococcus gordonii and Fusobacterium nucleatum biofilms . Material and Methods: Biofilms composed of two bacterial species, S. gordonii and F. nucleatum , were cultured for 1, 4, 7, and 10 days. The presence of both species was confirmed via amplification of the srtA and radD genes using real-time PCR. The concentrations of proteins associated with the biofilms and individual species were quantified using Western blotting. Results: The protein profiles of S. gordonii and F. nucleatum from individual cultures determined using one-dimensional electrophoresis revealed proteins found in S. gordonii and in F. nucleatum. Ct and reciprocal Ct values were determined for the exposed S. gordonii and F. nucleatum biofilms. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein was detected in biofilms and F. nucleatum , whereas HSP40 protein was present only in biofilms after 7 and 10 days of formation . Conclusion: HSP40 was detected only in the formed biofilms; thus, HSP40 is an essential proteins for adhesion. |
| Databáze: | OpenAIRE |
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