Expression of pro-inflammatory cytokines in rainbow trout (Oncorhynchus mykiss) during an infection with Ichthyophthirius multifiliis
| Autor: | K. Buchmann, J Sigh, Thomas Lindenstrøm |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Chemokine
DNA Complementary Time Factors medicine.medical_treatment Ciliophora Infections Spleen Aquatic Science Kidney Proinflammatory cytokine Fish Diseases medicine Animals Environmental Chemistry Interleukin 8 DNA Primers Hymenostomatida Skin Electrophoresis Agar Gel Ichthyophthirius multifiliis biology Reverse Transcriptase Polymerase Chain Reaction General Medicine biology.organism_classification medicine.anatomical_structure Cytokine Gene Expression Regulation Oncorhynchus mykiss Immunology biology.protein Cytokines Tumor necrosis factor alpha Rainbow trout |
| Zdroj: | University of Copenhagen |
| ISSN: | 1050-4648 |
| Popis: | Inflammatory processes are known to take place in the skin of fish during an infection with the parasitic ciliate Ichthyophthirius multifiliis. The present study shows that the pro-inflammatory cytokines interleukin 1 beta (IL-1beta) and tumor necrosis factor alpha (TNF-alpha), the chemokine interleukin 8 (IL-8) and the type II IL-1 receptor (IL-1RII) are expressed in rainbow trout during an infection. In the skin an up to 17.8 times increase in the expression level of IL-1beta relative to uninfected controls was seen 4 days after infection. Upregulated expression of IL-1beta was likewise observed in the head kidney and spleen during the infection although to a lesser degree. TNF-alpha expression was seen in all examined tissues, with an increase in transcript level in the skin of up to 3.2 times the level in uninfected fish. A significant increase in the expression of IL-8 was evident in the skin and also to a lower extent in the head kidney and spleen. Expression of the down regulatory IL-1RII was seen in all three tissues. The potential involvement of the expression of these genes and their relevance in an infection with I. multifiliis are discussed. |
| Databáze: | OpenAIRE |
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