Simultaneous analysis of nitrite, nitrate and the nicotinamide nucleotides by capillary electrophoresis: Application to biochemical studies and human extracellular fluids

Autor:	Paul G. Winyard, David R. Blake, David Perrett, Zhi Zhang, Bo R. Nielsen, Claire A. Davies
Rok vydání:	1999
Předmět:	Nicotinamide Clinical Biochemistry Nicotinamide adenine dinucleotide Nitrite reductase Biochemistry Analytical Chemistry Nitric oxide chemistry.chemical_compound Capillary electrophoresis chemistry Nitrate NAD+ kinase Nitrite
Zdroj:	ResearcherID
ISSN:	1522-2683 0173-0835
Popis:	A simple but rapid capillary electrophoresis method was developed for the measurement of nitrite and nitrate in human extracellular fluids and other aqueous solutions. The capabilities of the method were demonstrated by the measurement of endogenous nitrite and nitrate in plasma and serum samples from healthy volunteers, and serum and synovial fluid samples from rheumatoid arthritis patients. Furthermore, this method was used to simultaneously measure nicotinamide adenine dinucleotide, reduced (NADH), nicotinamide adenine dinucleotide (NAD+), nitrite, and nitrate, when studying the nitrite reductase activity of xanthine oxidase. The stability of nitrite was also investigated and it was found that when whole blood was spiked with nitrite and then processed, the nitrite was more stable in the plasma than in the serum. Our findings may help to explain the variations in basal nitrite concentrations reported in the literature.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c18042152e4dc419a9782d2460a5c54c https://doi.org/10.1002/(sici)1522-2683(19990701)20:10<2111::aid-elps2111>3.0.co Zobrazit plný text záznamu Plný text