Regulatory roles of claudin-1 in cell adhesion and microvilli formation
Autor: | Tomoyuki Aoyama, Taishi Akimoto, Daisuke Kyuno, Taro Murakami, Hiroshi Kitajima, Hiromu Suzuki, Kumi Takasawa, Makoto Osanai, Kazufumi Magara, Akira Takasawa, Yusuke Ono |
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Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
Microvilli Tight junction Chemistry Moesin Biophysics Cell Biology Biochemistry digestive system diseases Cell biology Focal adhesion 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Ezrin Radixin 030220 oncology & carcinogenesis Claudin-1 Proteome Cell Adhesion Tumor Cells Cultured Humans Cell adhesion Claudin Molecular Biology |
Zdroj: | Biochemical and Biophysical Research Communications. 565:36-42 |
ISSN: | 0006-291X |
Popis: | Aberrant expression of tight junction proteins has recently been focused on in the cancer research field. We previously showed that claudin-1 is aberrantly expressed from an early stage of uterine cervical adenocarcinoma and contributes to malignant potentials. To elucidate the molecular mechanisms underlying tumor-promoting roles of claudin-1, we established and analyzed claudin-1 knockout cells. Knockout of claudin-1 suppressed conventional tight junctional functions, barrier and fence functions, and expression of cell adhesion-associated proteins including E-cadherin. Comparative proteome analysis revealed that expression of claudin-1 affected expression of a wide range of proteins, especially proteins that are associated with cell adhesion and actin cytoskeleton remodeling. Interactome analysis of the identified proteins revealed that E-cadherin and focal adhesion kinase play central roles in the claudin-1-dependently affected protein network. Moreover, knockout of claudin-1 significantly suppressed microvilli formation and activity of Ezrin/Radixin/Moesin. Taken together, the results indicate that expression of claudin-1 affects not only conventional tight junction function but also expression and activity of a wide range of proteins, especially proteins that are associated with cell adhesion and actin cytoskeleton remodeling, to contribute to malignant potentials and microvilli formation in cervical adenocarcinoma cells. |
Databáze: | OpenAIRE |
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