Regulatory roles of claudin-1 in cell adhesion and microvilli formation

Autor: Tomoyuki Aoyama, Taishi Akimoto, Daisuke Kyuno, Taro Murakami, Hiroshi Kitajima, Hiromu Suzuki, Kumi Takasawa, Makoto Osanai, Kazufumi Magara, Akira Takasawa, Yusuke Ono
Rok vydání: 2021
Předmět:
Zdroj: Biochemical and Biophysical Research Communications. 565:36-42
ISSN: 0006-291X
Popis: Aberrant expression of tight junction proteins has recently been focused on in the cancer research field. We previously showed that claudin-1 is aberrantly expressed from an early stage of uterine cervical adenocarcinoma and contributes to malignant potentials. To elucidate the molecular mechanisms underlying tumor-promoting roles of claudin-1, we established and analyzed claudin-1 knockout cells. Knockout of claudin-1 suppressed conventional tight junctional functions, barrier and fence functions, and expression of cell adhesion-associated proteins including E-cadherin. Comparative proteome analysis revealed that expression of claudin-1 affected expression of a wide range of proteins, especially proteins that are associated with cell adhesion and actin cytoskeleton remodeling. Interactome analysis of the identified proteins revealed that E-cadherin and focal adhesion kinase play central roles in the claudin-1-dependently affected protein network. Moreover, knockout of claudin-1 significantly suppressed microvilli formation and activity of Ezrin/Radixin/Moesin. Taken together, the results indicate that expression of claudin-1 affects not only conventional tight junction function but also expression and activity of a wide range of proteins, especially proteins that are associated with cell adhesion and actin cytoskeleton remodeling, to contribute to malignant potentials and microvilli formation in cervical adenocarcinoma cells.
Databáze: OpenAIRE
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