Altered Skeletal Muscle Fatty Acid Handling in Subjects with Impaired Glucose Tolerance as Compared to Impaired Fasting Glucose
| Autor: | C.C.M. Moors, Ellen Konings, Ellen E. Blaak, Gijs H. Goossens, Michaela Diamant, Anneke Jans, Johan W. E. Jocken, Nynke J. van der Zijl |
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| Přispěvatelé: | Humane Biologie, RS: NUTRIM - HB/BW section A, RS: NUTRIM - R1 - Metabolic Syndrome, Promovendi NTM, Internal medicine, ICaR - Circulation and metabolism |
| Rok vydání: | 2016 |
| Předmět: |
Blood Glucose
Male 0301 basic medicine Time Factors Glucose uptake Palmitic Acid Impaired glucose tolerance 0302 clinical medicine impaired fasting glucose insulin resistance lipid metabolism Netherlands chemistry.chemical_classification Nutrition and Dietetics Fasting Middle Aged Glucose clamp technique medicine.anatomical_structure Liver Female lipids (amino acids peptides and proteins) lcsh:Nutrition. Foods and food supply hormones hormone substitutes and hormone antagonists medicine.medical_specialty lcsh:TX341-641 030209 endocrinology & metabolism Biology Gene Expression Regulation Enzymologic Article Diglycerides 03 medical and health sciences Insulin resistance Double-Blind Method Internal medicine Glucose Intolerance medicine Humans skeletal muscle Muscle Skeletal Triglycerides nutritional and metabolic diseases Skeletal muscle Fatty acid Metabolism medicine.disease Impaired fasting glucose impaired glucose tolerance 030104 developmental biology Endocrinology chemistry Glucose Clamp Technique Biomarkers Food Science |
| Zdroj: | Goossens, G H, Moors, C C M, Jocken, J W E, van der Zijl, N J, Jans, A, Konings, E, Diamant, M & Blaak, E E 2016, ' Altered Skeletal Muscle Fatty Acid Handling in Subjects with Impaired Glucose Tolerance as Compared to Impaired Fasting Glucose ', Nutrients, vol. 8, no. 3, 164 . https://doi.org/10.3390/nu8030164 Nutrients, 8(3). Multidisciplinary Digital Publishing Institute (MDPI) Nutrients; Volume 8; Issue 3; Pages: 164 Nutrients Nutrients, 8(3):164. Multidisciplinary Digital Publishing Institute (MDPI) Nutrients, Vol 8, Iss 3, p 164 (2016) |
| ISSN: | 2072-6643 |
| DOI: | 10.3390/nu8030164 |
| Popis: | Altered skeletal muscle fatty acid (FA) metabolism contributes to insulin resistance. Here, we compared skeletal muscle FA handling between subjects with impaired fasting glucose (IFG; n = 12 (7 males)) and impaired glucose tolerance (IGT; n = 14 (7 males)) by measuring arterio-venous concentration differences across forearm muscle. [H-2(2)]-palmitate was infused intravenously, labeling circulating endogenous triacylglycerol (TAG) and free fatty acids (FFA), whereas [U-C-13]-palmitate was incorporated in a high-fat mixed-meal, labeling chylomicron-TAG. Skeletal muscle biopsies were taken to determine muscle TAG, diacylglycerol (DAG), FFA, and phospholipid content, their fractional synthetic rate (FSR) and degree of saturation, and gene expression. Insulin sensitivity was assessed using a hyperinsulinemic-euglycemic clamp. Net skeletal muscle glucose uptake was lower (p = 0.018) and peripheral insulin sensitivity tended to be reduced (p = 0.064) in IGT as compared to IFG subjects. Furthermore, IGT showed higher skeletal muscle extraction of VLDL-TAG (p = 0.043), higher muscle TAG content (p = 0.025), higher saturation of FFA (p = 0.004), lower saturation of TAG (p = 0.017) and a tendency towards a lower TAG FSR (p = 0.073) and a lower saturation of DAG (p = 0.059) versus IFG individuals. Muscle oxidative gene expression was lower in IGT subjects. In conclusion, increased liver-derived TAG extraction and reduced lipid turnover of saturated FA, rather than DAG content, in skeletal muscle accompany the more pronounced insulin resistance in IGT versus IFG subjects. |
| Databáze: | OpenAIRE |
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