Mechanisms involved in the photosensitized inactivation ofAcanthamoeba palestinensistrophozoites
Autor: | Stefania Ferro, Giulio Jori, Giuseppe Tognon, Laura Guidolin, Olimpia Coppellotti |
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Rok vydání: | 2009 |
Předmět: |
trophozoites
Indoles Population Succinic Acid Acanthamoeba Vacuole Biology photosensitisation Applied Microbiology and Biotechnology tetracationic phthalocyanine Microscopy Electron Transmission Organometallic Compounds medicine Lactic Acid education Cell damage education.field_of_study Photosensitizing Agents L-Lactate Dehydrogenase Caspase 3 General Medicine Acanthamoeba palestinensis medicine.disease biology.organism_classification Mitochondria Contractile vacuole Cytosol Microscopy Fluorescence Photochemotherapy Biochemistry Spectrophotometry Cytoplasm Biophysics Biotechnology |
Zdroj: | Journal of Applied Microbiology. 107:1615-1623 |
ISSN: | 1365-2672 1364-5072 |
Popis: | Aims: To advance our understanding of the mechanisms involved in the RLP068 phthalocyanine-photosensitized inactivation of Acanthamoeba palestinensis trophozoites through a precise identification of the targets of the photoprocess in both the cytosolic and mitochondrial compartments. Methods and Results: We followed the activities of selected marker enzymes as well as we performed fluorescence and transmission electron microscopy investigations of the alterations induced by the photoprocess in the fine structure of subcellular compartments. RLP068 is preferentially located in the contractile vacuole: the fluorescence in that site is particularly evident in the unirradiated cells and becomes more diffused after irradiation. Electron microscopic analysis of photosensitized A. palestinensis cells clearly shows that the swelling of trophozoites and the appearance of vacuoles spread throughout the cytoplasm after phototreatment. The activity of a typical cytoplasmic enzyme, such as lactate dehydrogenase, underwent a 35% decrease as a consequence of the photoprocess, reflecting the photodamage induced by migrating phthalocyanine molecules in their micro-environment. Conclusions: The presence of multiple targets for the phthalocyanine-photosensitized process is of utmost importance because this pattern of cell damage makes it unlikely that photoresistant A. palestinensis strains are gradually selected or mutagenic phenomena are developed as a consequence of the photoinduced damage. Significance and Impact of the Study: Photosensitization via phthalocyanines appears to represent an efficient and safe approach for achieving a close control of the population of a potentially pathogenic protozoan such as A. palestinensis, opening new perspectives for the disinfection of microbiologically polluted waters. |
Databáze: | OpenAIRE |
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