Essential fatty acid deficiency induces fatty acid desaturase expression in rat epididymis, but not in testis
| Autor: | Thomas Sæther, Trine B. Haugen, Helge Rootwelt, Bjørn O. Christophersen, Thien N Tran, Hans J Grav |
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| Rok vydání: | 2007 |
| Předmět: |
Fatty Acid Desaturases
Male Embryology medicine.medical_specialty Chromatography Gas Enzyme activator Endocrinology Downregulation and upregulation Internal medicine Testis Gene expression medicine Animals RNA Messenger Rats Wistar Epididymis chemistry.chemical_classification Arachidonic Acid Sertoli Cells Fatty Acids Essential biology Obstetrics and Gynecology Fatty acid RNA Lipid metabolism Cell Biology Lipid Metabolism Lipids Spermatozoa Diet Rats Enzyme Activation medicine.anatomical_structure Fatty acid desaturase Liver Reproductive Medicine chemistry biology.protein Stearoyl-CoA Desaturase |
| Zdroj: | Reproduction. 133:467-477 |
| ISSN: | 1741-7899 1470-1626 |
| DOI: | 10.1530/rep-06-00294 |
| Popis: | On the molecular level, essential fatty acid deficiency (EFAD) has been associated with induced fatty acid (FA) desaturase expression and activity in several tissues. However, there seem to be exceptions. In the present study, we examine the effects of EFAD in the male rat genital tract, combining FA analysis, gene expression studies, and morphological evaluation of epididymal spermatozoa. When feeding 21-day-old Wistar rats, a fat-free diet for 6 weeks, an increase in 18:1n-9 and 20:3n-9 and a concomitant decrease in the 18:2n-6 and 20:4n-6 species are seen in testis, as well as in liver. However, with regard to desaturase expression the rat testis seems to be unresponsive to EFAD conditions, in contrast to other organs studied. In the sexually mature testis none of the desaturases (SCD1, SCD2, D5D, or D6D) are induced in response to lowered contents of polyunsaturated FAs. This also applies tocaputepididymis, while EFAD sensitivity is regained incaudaepididymis, where the desaturases are upregulated. The FA profile of epididymal spermatozoa is increasingly affected by EFAD during the transport from testis tocaudaepididymis. Furthermore, a significant increase in the number of abnormal spermatozoa is observed incaudaepididymis. |
| Databáze: | OpenAIRE |
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