A Comparison of Four Molecular Methods for Detection of Aflatoxin-Producing Aspergillus in Peanut and Dried Shrimp Samples Collected from Local Markets around Pathum Thani Province, Thailand
| Autor: | Panan Kanchanaphum, Ratchanok Kumsiri |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Aflatoxin
Veterinary medicine Article Subject Science Loop-mediated isothermal amplification Aspergillus flavus Fungus 01 natural sciences Conidium 03 medical and health sciences food Dried shrimp General Environmental Science 0303 health sciences Aspergillus biology 030306 microbiology fungi 010401 analytical chemistry food and beverages Contamination biology.organism_classification food.food 0104 chemical sciences Medicine General Agricultural and Biological Sciences |
| Zdroj: | Scientifica, Vol 2020 (2020) |
| ISSN: | 2090-908X |
| DOI: | 10.1155/2020/8580451 |
| Popis: | Aspergillus flavus is an aflatoxin-producing fungus which is poisonous to humans and animals when consumed. Detecting the fungus can help to prevent this danger. The four molecular methods, namely, conventional isothermal amplification (LAMP), PCR, quantitative LAMP (qLAMP), and qPCR, were compared to determine their efficiency for A. flavus detection. Thirty samples of peanut and dried shrimp were collected from 15 markets around Pathum Thani Province in Thailand. The samples were artificially infected with 108 conidia/ml of A. flavus for 1 hr and enriched for one day to represent real contamination. The results show that the sensitivity detection for A. flavus in PCR, LAMP, qPCR, and qLAMP was 50 ng, 5 ng, 5 pg, and 5 pg, respectively. Aspergillus in 30 peanut and dried shrimp from the market was detected by all four methods. The detection rate was about 20%, 60%, 100%, and 100% with PCR, LAMP, qPCR, and qLAMP, respectively. The molecular detection technique, especially LAMP, qPCR, and qLAMP, can detect this pathogenic fungi very rapidly with high sensitivity and reliability in comparison to conventional PCR. |
| Databáze: | OpenAIRE |
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