Efficient entry of budded virions of Autographa californica multiple nucleopolyhedrovirus into Spodoptera frugiperda cells is dependent on dynamin, Rab5, and Rab11
| Autor: | Zhaofei Li, Gary W. Blissard, Fanye Yan, Ximeng Liu, Jingfeng Li, Ya Guo, Qi Yue |
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| Rok vydání: | 2020 |
| Předmět: |
Dynamins
0106 biological sciences food.ingredient Endosome viruses Sf9 Endosomes Biology Spodoptera Virus Replication Endocytosis 01 natural sciences Biochemistry Cell Line 03 medical and health sciences food Sf9 Cells Animals Molecular Biology Virus Release rab5 GTP-Binding Proteins 030304 developmental biology Dynamin 0303 health sciences Host Microbial Interactions fungi DNA virus Virus Internalization biochemical phenomena metabolism and nutrition biology.organism_classification Nucleopolyhedroviruses Cell biology Lepidoptera 010602 entomology Autographa californica Alphabaculovirus rab GTP-Binding Proteins Insect Science RNA Interference |
| Zdroj: | Insect Biochemistry and Molecular Biology. 123:103409 |
| ISSN: | 0965-1748 |
| DOI: | 10.1016/j.ibmb.2020.103409 |
| Popis: | Autographa californica multiple nucleopolyhedrovirus (AcMNPV), a member of the Alphabaculovirus genus of the family Baculoviridae, is an enveloped double-stranded DNA virus. Budded virions (BVs) of AcMNPV enter host cells via clathrin-mediated endocytosis. However, the route of functional intracellular trafficking of AcMNPV BVs during entry is not well established. In the current study, we found that entering BVs were colocalized mainly with cellular Rab5 and Rab11. Expression of dominant-negative (DN) Rab5 and Rab11 or RNAi-mediated down regulation of these two cellular transcripts significantly reduced BVs entry into but not egress from Spodoptera frugiperda cells (Sf9), whereas similar treatments for Rab4 and Rab7 had no apparent effect on virus infection. Combined with data from RNAi knockdowns of dynamin, and dynasore inhibition assays, our results support a model in which AcMNPV BVs enter permissive host cells by clathrin-mediated endocytosis, followed by de-envelopment of BVs predominantly within early and maturing endosomes rather than within late endosomes. Additionally, Rab11 suppression studies suggest the Rab11-dependent recycling endosomal pathway is involved in virion entry. |
| Databáze: | OpenAIRE |
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