Identification and characterization of genes induced during sexual differentiation in Schizosaccharomyces pombe
| Autor: | Shusei Sato, Satoshi Tabata, Utut Widyastuti, Hideki Suzuki, Yasuo Hotta |
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| Rok vydání: | 1994 |
| Předmět: |
DNA
Complementary Sex Differentiation Amino Acid Transport Systems Genes Fungal Molecular Sequence Data Saccharomyces cerevisiae Homology (biology) Fungal Proteins Gene Expression Regulation Fungal Complementary DNA Schizosaccharomyces Gene expression Genetics Amino Acid Sequence DNA Fungal Gene Sequence Homology Amino Acid biology cDNA library Cell Cycle Serine Endopeptidases Membrane Transport Proteins General Medicine Spores Fungal biology.organism_classification Molecular biology Schizosaccharomyces pombe Schizosaccharomyces pombe Proteins Carrier Proteins Sequence Alignment |
| Zdroj: | Current Genetics. 26:31-37 |
| ISSN: | 1432-0983 0172-8083 |
| DOI: | 10.1007/bf00326301 |
| Popis: | Five cDNA clones, harboring genetic messages preferentially expressed during the sexual differentiation process, were isolated from a cDNA library of Schizosaccharomyces pombe by subtractive screening. Transcription of the corresponding genes, termed isp3, 4, 5, 6, and 7, was dependent on nitrogen starvation and their induction occurred at several stages of spore formation. Analysis of the cDNA primary structures revealed a capacity for the coding of polypeptides of 19.2 kDa, 88.3 kDa, 60.1 kDa, 49.7 kDa, and 43.8 kDa, respectively. The translated amino-acid sequences of isp5 and isp6 were found to show significant similarities to those of amino-acid permeases and proteinase B of Saccharomyces cerevisiae, respectively. Disruption of isp6 arrested the cell cycle prior to conjugation and caused a drastic blocking effect on spore formation. |
| Databáze: | OpenAIRE |
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