A cost-effective carbohydrate fermentation test for yeast using microtitre plate
| Autor: | Pravin M. V. Charles, Sreenivasan Srirangaraj, Arunava Kali |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Microbiology (medical)
carbohydrate fermentation test Time Factors Cost-Benefit Analysis Positive reaction lcsh:QR1-502 yeast identification Sensitivity and Specificity lcsh:Microbiology Microbiology Incubation period Yeasts Carbohydrate fermentation test Carbohydrate fermentation Humans Food science Mycological Typing Techniques Candida biology Microtitre plate miniaturized biochemical tests biology.organism_classification Yeast Mycoses Fermentation Carbohydrate Metabolism Bacteria |
| Zdroj: | Indian Journal of Medical Microbiology, Vol 33, Iss 2, Pp 293-295 (2015) |
| ISSN: | 1998-3646 0255-0857 |
| Popis: | Carbohydrate fermentation test is a well-established technique for speciation of bacteria and fungi. However, long incubation period, cost and procedural complexity may limit its use. Here, we describe a simple modification of conventional carbohydrate fermentation technique using microtitre plate. Thirty-one yeast isolates were identified based on their fermentation property by microtitre plate method and results were compared with conventional method. The modified method had 85.7% sensitivity and 100% specificity. The average time taken to produce positive reaction was 24 hours. When compared with conventional method, modified method reduced turn-around time and cost of processing without significant increase in discordant results. |
| Databáze: | OpenAIRE |
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