Enzymatic Reconstitution and Biosynthetic Investigation of the Bacterial Carbazole Neocarazostatin A
| Autor: | Qing Fang, Li Su, Kirstie A. Rickaby, Xiaohui Yang, Yating Liu, Zixin Deng, Hai Deng, Jioji N. Tabudravu, Kwaku Kyeremeh, Laurent Trembleau, Yi Yu |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
chemistry.chemical_classification
Supplementary data Molecular Structure 010405 organic chemistry Chemistry Carbazole F410 Metabolite Organic Chemistry Carbazoles Computational Biology 010402 general chemistry 01 natural sciences Streptomyces 0104 chemical sciences chemistry.chemical_compound Polyketide Enzyme Biochemistry Biosynthesis Cytochrome P-450 Enzyme System Transferases |
| ISSN: | 0022-3263 |
| Popis: | Tricyclic carbazole is an important scaffold in many naturally occurring metabolites, as well as valuable building blocks. Here we report the reconstitution of the ring A formation of the bacterial neocarazostatin A carbazole metabolite. We provide evidence of the involvement of two unusual aromatic polyketide proteins. This finding suggests how new enzymatic activities can be recruited to specific pathways to expand biosynthetic capacities. Finally, we leveraged our bioinformatics survey to identify the untapped capacity of carbazole biosynthesis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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