Expression and Function of ATIP/MTUS1 in Human Prostate Cancer Cell Lines
| Autor: | Simon N S Louis, Albert G Frauman, William J. Louis, Laurie T C Chow, Michael A Krezel, Linda Adriana Rezmann, Kevin J. Catt, Christos Tikellis |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Male
medicine.medical_specialty Urology Polymerase Chain Reaction Article Prostate cancer Growth factor receptor Epidermal growth factor Internal medicine Cell Line Tumor LNCaP Medicine Humans RNA Messenger RNA Small Interfering DNA Primers Epidermal Growth Factor business.industry Cell growth Tumor Suppressor Proteins Cancer Prostatic Neoplasms medicine.disease Gene Expression Regulation Neoplastic Endocrinology Oncology Cell culture Cancer research Signal transduction business hormones hormone substitutes and hormone antagonists Thymidine |
| Popis: | BACKGROUND We have previously demonstrated Ang II type 2 (AT2-) receptor-mediated inhibition of EGF-induced prostate cancer cell growth in androgen-dependent (LNCaP) and independent (PC3) prostate cancer cell lines. METHODS To explore the signaling pathways involved in this inhibitory effect, we examined the interaction of the AT2-receptor with its novel regulatory partner ATIP using real time PCR, over-expression, siRNA and [3H]thymidine incorporation assays. RESULTS The results in human prostate cancer cell lines demonstrate the presence of ATIP in both cell lines examined, and suggest that (i) the AT2-receptor through an interaction with ATIP mediates an anti-growth factor effect in both androgen-dependent and androgen-independent cell lines; (ii) ATIP expression decreases as the rate of cell growth and androgen-independence increase; and (iii) EGF may act on cell growth in part by reducing the content of ATIP present in the cells. CONCLUSIONS The results support our earlier proposal in normal cell lines that ATIP is an important component of the cellular response to AT2-receptor activation. The results further suggest that a critical level of ATIP is required to mediate the effect of AT2-receptor activation to inhibit EGF mediated increases in cell growth. They also suggest that EGF may in part induce cell growth by suppressing the level of ATIP expression. Prostate 70: 1563–1574, 2010. © 2010 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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