The variable fine structure of elastin visualized with verhoeff's iron hematoxylin
| Autor: | R. M. Brissie, N. T. Thompson, Samuel S. Spicer |
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| Rok vydání: | 1975 |
| Předmět: |
Male
Lamina Pathology medicine.medical_specialty Iron Guinea Pigs Mice Fetus Adventitia Testis medicine Animals Benzopyrans Citrates Mast Cells Fibroblast Lamina propria Tracheal Epithelium Staining and Labeling biology Chemistry Cartilage Anatomy Fibroblasts respiratory system Elastic Tissue Agricultural and Biological Sciences (miscellaneous) Elastin Trachea Microscopy Electron medicine.anatomical_structure Indenes Lead Ultrastructure biology.protein Female Collagen |
| Zdroj: | The Anatomical Record. 181:83-94 |
| ISSN: | 1097-0185 0003-276X |
| DOI: | 10.1002/ar.1091810107 |
| Popis: | Verhoeff's iron hematoxylin (VIH) followed by lead citrate (LC) applied to epoxy thin sections stained the dense component of elastic fibers heavily and the peripheral microfibrillar component lightly in guinea pig trachea and mouse testis fixed with a glutaraldehyde-osmium tetroxide sequence. This method stained large fimbriated fibers beneath tracheal epithelium, small fibers and stacked aggregates thereof in the deep lamina propria, cartilage and adventitia of the trachea and large stacked fibers in the fibroelastic band of the trachea. Fibers of the fetus differed from those of the adult, especially in the subepithelial elastic lamina of the trachea. Elastic fibers were intimately associated with fibroblasts and particularly slender fibroblast processes in tracheal stroma and with chondrocytes in tracheal cartilage. Fibroblasts associated with elastic fibers in the tracheal subepithelial lamina propria were often closely bordered by eosinophils, mast cells, or monocytes. Occasional mast cells extended slender processes around elastic fibers in the subepithelial lamina propria. In mouse testis and in many regions of the trachea, small elastic fibers were identified which were below the limits of resolution for the light microscope and were not apparent at the ultrastructural level in routinely stained thin sections. |
| Databáze: | OpenAIRE |
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