Proliferating cell nuclear antigen associates to protein complexes containing cyclins/cyclin dependent kinases susceptible of inhibition by KRPs during maize germination
| Autor: | Elpidio García-Ramírez, Estefany Damaris Guerrero-Molina, Jorge M. Vázquez-Ramos, Aldo Tonatiuh Lemus-Enciso, Javier Axosco-Marín, Aurora Lara-Núñez, Sara M. Garza-Aguilar |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Cell Cycle Proteins Germination Plant Science Zea mays 01 natural sciences 03 medical and health sciences Cyclin-dependent kinase Cyclins Proliferating Cell Nuclear Antigen Genetics Phosphorylation Kinase activity Cell Cycle Protein Plant Proteins Cyclin chemistry.chemical_classification biology Kinase General Medicine Cell cycle Cyclin-Dependent Kinases Proliferating cell nuclear antigen Cell biology 030104 developmental biology Enzyme chemistry biology.protein Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Plant Science. 280:297-304 |
| ISSN: | 0168-9452 |
| DOI: | 10.1016/j.plantsci.2018.12.020 |
| Popis: | The Proliferating Cell Nuclear Antigen, PCNA, has roles in both G1 and S phases of the cell cycle. Here we show that maize PCNA can be found in cells in structures of a trimer or a dimer of trimer, in complexes of high molecular mass that change in size as germination proceeds, co-eluting with cell cycle proteins as CycD3;1 and CDKs (A/B1;1). Using different methodological strategies, we show that PCNA actually interacts with CycD3;1, CDKA, CDKB1;1, KRP1;1 and KRP4;1, all of which contain PIP or PIP-like motifs. Anti-PCNA immunoprecipitates show kinase activity that is inhibited by KRP1;1 and KRP4;2, indicating the formation of quaternary complexes PCNA-CycD/CDKs-KRPs in which PCNA would act as a platform. This inhibitory effect seems to be differential during the germination process, more pronounced as germination advances, suggesting a complex regulatory mechanism in which PCNA could bind different sets of cyclins/CDKs, some more susceptible to inhibition by KRPs than others. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect