Peroxisome proliferator-activated receptor β activation promotes myonuclear accretion in skeletal muscle of adult and aged mice

Autor: Anne-Sophie Rousseau, Nicole Wagner, Christian Giordano, Brigitte Sibille, Paul Grimaldi, Joseph Murdaca, Chantal Jehl-Pietri, Céline Gaudel, Pascal Lopez
Rok vydání: 2009
Předmět:
Male
Aging
medicine.medical_specialty
Satellite Cells
Skeletal Muscle
Cell division
Physiology
Calcineurin Inhibitors
Muscle Fibers
Skeletal
Clinical Biochemistry
Molecular and Genomic Physiology
Peroxisome proliferator-activated receptor
Mice
Inbred Strains
Mice
Transgenic
Citrate (si)-Synthase
Biology
Cell Fusion
Myoblasts
Mice
Proliferating Cell Nuclear Antigen
Skeletal muscle fibre
Physiology (medical)
Internal medicine
Precursor cell
medicine
Animals
Myocyte
Muscle
Skeletal
Receptor
PPAR-beta
Cell Nucleus
chemistry.chemical_classification
NFATC Transcription Factors
Cell growth
Calcineurin
Myogenic response
Skeletal muscle
Hedgehog signaling pathway
Mice
Inbred C57BL
Succinate Dehydrogenase
Thiazoles
Endocrinology
medicine.anatomical_structure
chemistry
Muscle plasticity
Muscle Fibers
Fast-Twitch
Cyclosporine
Cell Division
Zdroj: Pflugers Archiv
ISSN: 1432-2013
0031-6768
DOI: 10.1007/s00424-009-0676-9
Popis: We reported recently that peroxisome proliferator-activated receptor beta (PPARbeta) activation promotes a calcineurin-dependent exercise-like remodelling characterised by increased numbers of oxidative fibres and capillaries. As physical exercise also induces myonuclear accretion, we investigated whether PPARbeta activation alters myonuclear density. Transgenic muscle-specific PPARbeta over-expression induced 14% increase of myonuclear density. Pharmacological PPARbeta activation promoted rapid and massive myonuclear accretion (20% increase after 48 h), which is dependent upon calcineurin/nuclear factor of activated T cells signalling pathway. In vivo bromodeoxyuridine labelling and proliferating cell nuclear antigen immunodetection revealed that PPARbeta activation did not promote cell proliferation, suggesting that the PPARbeta-promoted myonuclear accretion involves fusion of pre-existing muscle precursor cells to myofibres rather than cell division. Finally, we showed that in skeletal muscle, ageing led to a down-regulation of PPARbeta accompanied by decrease of both oxidative fibre number and myonuclear density. PPARbeta pharmacological activation counteracts, at least in part, the ageing-driven muscle remodelling.
Databáze: OpenAIRE
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