PACLOBUTRAZOL-RESISTANCE Gene Family Regulates Floral Organ Growth with Unequal Genetic Redundancy in Arabidopsis thaliana
| Autor: | Eunsun Kim, Inhye Lee, Kihye Shin, Soon Ki Park, Sumin Lee, Moon-Soo Soh |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Arabidopsis thaliana Sterility Vegetative reproduction Mutant Stamen Biology 01 natural sciences Catalysis Article Paclobutrazol Inorganic Chemistry lcsh:Chemistry 03 medical and health sciences chemistry.chemical_compound Gene family Physical and Theoretical Chemistry Molecular Biology Gene lcsh:QH301-705.5 Spectroscopy fertility Organic Chemistry food and beverages General Medicine Computer Science Applications Cell biology 030104 developmental biology chemistry lcsh:Biology (General) lcsh:QD1-999 Genetic redundancy unequal redundancy autogamous reproduction PRE 010606 plant biology & botany |
| Zdroj: | International Journal of Molecular Sciences, Vol 20, Iss 4, p 869 (2019) International Journal of Molecular Sciences Volume 20 Issue 4 |
| ISSN: | 1422-0067 |
| Popis: | A PACLOBUTRAZOL-RESISTANCE (PRE) gene family, consisting of six genes in Arabidopsis thaliana, encodes a group of helix-loop-helix proteins that act in the growth-promoting transcriptional network. To delineate the specific role of each of the PRE genes in organ growth, we took a reverse genetic approach by constructing high order pre loss-of-function mutants of Arabidopsis thaliana. In addition to dwarf vegetative growth, some double or high order pre mutants exhibited defective floral development, resulting in reduced fertility. While pre2pre5 is normally fertile, both pre2pre6 and pre5pre6 showed reduced fertility. Further, the reduced fertility was exacerbated in the pre2pre5pre6 mutant, indicative of the redundant and critical roles of these PREs. Self-pollination assay and scanning electron microscopy analysis showed that the sterility of pre2pre5pre6 was mainly ascribed to the reduced cell elongation of anther filament, limiting access of pollens to stigma. We found that the expression of a subset of flower-development related genes including ARGOS, IAA19, ACS8, and MYB24 was downregulated in the pre2pre5pre6 flowers. Given these results, we propose that PREs, with unequal functional redundancy, take part in the coordinated growth of floral organs, contributing to successful autogamous reproduction in Arabidopsis thaliana. |
| Databáze: | OpenAIRE |
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