Antifungal activity of well-defined chitooligosaccharide preparations against medically relevant yeasts

Autor: Peter Gaustad, Morten Sørlie, Silje Benedicte Lorentzen, Oddmund Bakke, Berit Bjugan Aam, Svein Halvor Knutsen, Jane Wittrup Agger, Vincent G. H. Eijsink, M. Ganan, Catherine Anne Heyward
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Antifungal Agents
Cell Membranes
Drug Evaluation
Preclinical
Oligosaccharides
Yeast and Fungal Models
Degree of polymerization
Pathology and Laboratory Medicine
Polymerization
Chitosan
chemistry.chemical_compound
Yeasts
Medicine and Health Sciences
Candida albicans
Candida
Fungicides
chemistry.chemical_classification
Fungal Pathogens
Multidisciplinary
biology
Molecular Structure
Antimicrobials
Fungal Diseases
Chemical Reactions
Drugs
Eukaryota
Agriculture
Oligosaccharide
Chemistry
Infectious Diseases
Biochemistry
Experimental Organism Systems
Medical Microbiology
Physical Sciences
Cell disruption
Medicine
Pathogens
Cellular Structures and Organelles
Agrochemicals
Research Article
Imaging Techniques
Science
030106 microbiology
Mycology
Microbial Sensitivity Tests
Research and Analysis Methods
Microbiology
03 medical and health sciences
Structure-Activity Relationship
Microbial Control
Fluorescence Imaging
Candida Albicans
Humans
Microbial Pathogens
Pharmacology
Antifungals
Organisms
Fungi
Biology and Life Sciences
Cell Biology
biology.organism_classification
Polymer Chemistry
Yeast
Molecular Weight
030104 developmental biology
Yeast Infections
chemistry
Mycoses
Solubility
Acetylation
Animal Studies
Zdroj: PLoS ONE
PLoS ONE, Vol 14, Iss 1, p e0210208 (2019)
ISSN: 1932-6203
Popis: Due to their antifungal activity, chitosan and its derivatives have potential to be used for treating yeast infections in humans. However, to be considered for use in human medicine, it is necessary to control and know the chemical composition of the compound, which is not always the case for polymeric chitosans. Here, we analyze the antifungal activity of a soluble and well-defined chito-oligosaccharide (CHOS) with an average polymerization degree (DPn) of 32 and fraction of acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains. Minimal inhibitory concentrations (MIC) varied widely among yeast species, strains and isolates (from > 5000 to < 9.77 μg mL-1) and inhibition patterns showed a time- and dose-dependencies. The antifungal activity was predominantly fungicidal and was inversely proportional to the pH, being maximal at pH 4.5, the lowest tested pH. Furthermore, antifungal effects of CHOS fractions with varying average molecular weight indicated that those fractions with an intermediate degree of polymerization, i.e. DP 31 and 54, had the strongest inhibitory effects. Confocal imaging showed that C32 adsorbs to the cell surface, with subsequent cell disruption and accumulation of C32 in the cytoplasm. Thus, C32 has potential to be used as a therapy for fungal infections.
Databáze: OpenAIRE
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