The microfollicle: a model of the human hair follicle for in vitro studies
| Autor: | Thi Phuong Tao, Tobias Lam, Scott Youngquist, Gerd Lindner, Beren Atac, Isabel Rütschle, Uwe Marx, Reza P. Azar, Teresa DiColandrea, Ping Hu, Beatrix Fauler, Clemens Edler, Roland Lauster, Marian Jädicke, Thorsten Mielke, Flora Marta Kiss |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Vascular Endothelial Growth Factor A Biology Outer root sheath Models Biological 03 medical and health sciences 0302 clinical medicine Gene expression Keratin medicine Organoid Humans Cells Cultured chemistry.chemical_classification integumentary system Lineage markers Infant Newborn Cell Biology General Medicine Hair follicle Cell biology 030104 developmental biology medicine.anatomical_structure Dermal papillae chemistry Gene Expression Regulation Minoxidil 030220 oncology & carcinogenesis Keratins Melanocytes Hair Follicle Biomarkers Developmental Biology medicine.drug |
| Zdroj: | In vitro cellulardevelopmental biology. Animal. 56(10) |
| ISSN: | 1543-706X |
| Popis: | Access to complex in vitro models that recapitulate the unique markers and cell-cell interactions of the hair follicle is rather limited. Creation of scalable, affordable, and relevant in vitro systems which can provide predictive screens of cosmetic ingredients and therapeutic actives for hair health would be highly valued. In this study, we explore the features of the microfollicle, a human hair follicle organoid model based on the spatio-temporally defined co-culture of primary cells. The microfollicle provides a 3D differentiation platform for outer root sheath keratinocytes, dermal papilla fibroblasts, and melanocytes, via epidermal-mesenchymal-neuroectodermal cross-talk. For assay applications, microfollicle cultures were adapted to 96-well plates suitable for medium-throughput testing up to 21 days, and characterized for their spatial and lineage markers. The microfollicles showed hair-specific keratin expression in both early and late stages of cultivation. The gene expression profile of microfollicles was also compared with human clinical biopsy samples in response to the benchmark hair-growth compound, minoxidil. The gene expression changes in microfollicles showed up to 75% overlap with the corresponding gene expression signature observed in the clinical study. Based on our results, the cultivation of the microfollicle appears to be a practical tool for generating testable insights for hair follicle development and offers a complex model for pre-clinical substance testing. |
| Databáze: | OpenAIRE |
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