The impact of acute stress disorder on gallbladder interstitial cells of Cajal
Autor: | Hu Qiu, Wei‐bo Chao, Zhen-Peng Huang, Hang Chen, Yue Liu, Hao‐bin Zhu, Bao-ping Yu, Ke Wang |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Physiology Clinical Biochemistry Motility Apoptosis Stem cell factor 03 medical and health sciences symbols.namesake 0302 clinical medicine Ovarian Follicle Western blot Gene expression Animals Medicine Stress Disorders Traumatic Acute medicine.diagnostic_test business.industry Gallbladder Cell Biology Interstitial Cells of Cajal Immunohistochemistry Interstitial cell of Cajal 030104 developmental biology Terminal deoxynucleotidyl transferase 030220 oncology & carcinogenesis symbols Female Rabbits Gastrointestinal Motility business Signal Transduction |
Zdroj: | Journal of Cellular Physiology. 235:8424-8431 |
ISSN: | 1097-4652 0021-9541 |
Popis: | Physical and psychological stress exerts a substantial effect on gastrointestinal motility disorders, where trauma enhances symptoms of digestive dysfunction. Interstitial cells of Cajal (ICCs) act as pacemakers for gastrointestinal motility regulation and are likely important in stress-associated gastrointestinal motility disorders. This study explored the mechanisms underlying gallbladder ICCs function under acute stress conditions using a rabbit chest puncture and cholecystectomy model. The stem cell factor (SCF)/c-kit pathway is essential for the development of ICCs, and gene expression was investigated to identify stress-induced transcriptional alterations. Immunohistochemistry, terminal deoxynucleotidyl transferase dUTP nick end labeling assays were used to determine ICCs apoptosis, whereas western blot analysis and reverse-transcription polymerase chain reaction were used to detect changes in the SCF/c-kit signaling pathway. These methods revealed a reduction in ICCs via apoptosis following stress, and ICCs increased over time after stressor removal. Therefore, this study demonstrates the impact of stress on ICCs development and survival and further confirms the link between stress and gastrointestinal motility. |
Databáze: | OpenAIRE |
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