Adipose-Derived Stem Cell Exosomes Promoted Hair Regeneration
| Autor: | Yue Li, Ningwen Zhu, Sainan Wu, Xiansheng Zhao, Jinyan Wu, Wenyu Wu, Ruoyue Yuan, Quyang Yang |
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| Rok vydání: | 2021 |
| Předmět: |
Vascular Endothelial Growth Factor A
0206 medical engineering Biomedical Engineering Mice Nude Medicine (miscellaneous) Adipose tissue 02 engineering and technology Biology Exosomes Exosome Andrology Mice 03 medical and health sciences chemistry.chemical_compound medicine Animals Regeneration 030304 developmental biology 0303 health sciences integumentary system Stem Cells Regeneration (biology) Hair follicle 020601 biomedical engineering Mice Inbred C57BL Vascular endothelial growth factor Transplantation medicine.anatomical_structure Dermal papillae chemistry Original Article Stem cell |
| Zdroj: | Tissue Eng Regen Med |
| ISSN: | 2212-5469 1738-2696 |
| Popis: | BACKGROUND: Some scholars have found that dermal papilla spheroid–derived exosomes could promote the development of hair follicles. However, whether adipose-derived stem cell exosomes (ADSC-Exos) have a similar effect on hair growth has not been determined yet. Thus, the purpose of this article was to detect whether ADSC-Exos could promote hair regeneration. METHODS: Adipose-derived stem cells (ADSCs) were isolated from 6-week-old C57BL/6 mice. Then, ADSC-Exos were isolated from the ADSCs. Western blotting was used to detect specific exosome markers. The particle size and distribution of the exosomes were analyzed by NanoSight dynamic light scattering. A total of 12 nude mice were randomly divided into two groups (n = 6 each): the ADSC-Exos group and the control group. For the control group, a mixture of freshly isolated dermal cells (DCs) and epidermal cells (ECs) was grafted. For the ADSC-Exos group, a mixture of DCs, ECs, and 50 μg/ml of ADSC-Exos was grafted. Gross evaluation of the hair regeneration was carried out 2–3 weeks after the transplantation, and the graft site was harvested for histology at the third week. RESULTS: The existence of exosomes derived from ADSCs was evidenced by CD63, ALX1, and CD9 expression. Two or three weeks after the grafting, the number of regenerated hairs in the ADSC-Exos group was higher than that in the control group (p |
| Databáze: | OpenAIRE |
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