Copper-Induced Topology Switching and Thrombin Inhibition with Telomeric DNA G-Quadruplexes
| Autor: | Guido H. Clever, David M. Engelhard, Julia Nowack |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Serine Proteinase Inhibitors
Stereochemistry Aptamer Supramolecular chemistry 010402 general chemistry Antiparallel (biochemistry) G-quadruplex 01 natural sciences Catalysis chemistry.chemical_compound Induced topology heterocyclic compounds biology Molecular Structure 010405 organic chemistry Hydrolysis Tetrahymena Thrombin Fibrinogen General Chemistry DNA Protozoan Telomere biology.organism_classification 0104 chemical sciences G-Quadruplexes chemistry Intramolecular force DNA Copper |
| Zdroj: | Angewandte Chemie (International ed. in English). 56(38) |
| ISSN: | 1521-3773 |
| Popis: | The topological diversity of DNA G-quadruplexes may play a crucial role in its biological function. Reversible control over a specific folding topology was achieved by the synthesis of a chiral, glycol-based pyridine ligand and its fourfold incorporation into human telomeric DNA by solid-phase synthesis. Square-planar coordination to a CuII ion led to the formation of a highly stabilizing intramolecular metal-base tetrad, substituting one G-tetrad in the parent unimolecular G-quadruplex. For the Tetrahymena telomeric repeat, CuII -triggered switching from a hybrid-dominated conformer mixture to an antiparallel topology was observed. CuII -dependent control over a protein-G-quadruplex interaction was shown for the thrombin-tba pair (tba=thrombin-binding aptamer). |
| Databáze: | OpenAIRE |
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