Structure of the C. elegans ZYG-1 Cryptic Polo Box Suggests a Conserved Mechanism for Centriolar Docking of Plk4 Kinases
| Autor: | Valeria Viscardi, Dmitri I. Svergun, Adam Round, Karen Oegema, Renping Qiao, Molly M. Lettman, Johannes Lesigang, Gang Dong, Ekaterina Shimanovskaya |
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| Rok vydání: | 2014 |
| Předmět: |
Models
Molecular PLK4 Centriole Molecular Sequence Data Plasma protein binding Protein Serine-Threonine Kinases Biology Structure-Activity Relationship Protein structure Species Specificity Structural Biology Animals Amino Acid Sequence Caenorhabditis elegans Caenorhabditis elegans Proteins Molecular Biology Peptide sequence Centrioles Acidic Region Protein Structure Tertiary Cell biology surgical procedures operative Biochemistry Docking (molecular) Dimerization Protein Kinases Protein Binding Centriole assembly |
| Zdroj: | Structure. 22:1090-1104 |
| ISSN: | 0969-2126 |
| Popis: | SummaryPlk4 family kinases control centriole assembly. Plk4s target mother centrioles through an interaction between their cryptic polo box (CPB) and acidic regions in the centriolar receptors SPD-2/Cep192 and/or Asterless/Cep152. Here, we report a crystal structure for the CPB of C. elegans ZYG-1, which forms a Z-shaped dimer containing an intermolecular β sheet with an extended basic surface patch. Biochemical and in vivo analysis revealed that electrostatic interactions dock the ZYG-1 CPB basic patch onto the SPD-2-derived acidic region to promote ZYG-1 targeting and new centriole assembly. Analysis of a different crystal form of the Drosophila Plk4 (DmPlk4) CPB suggests that it also forms a Z-shaped dimer. Comparison of the ZYG-1 and DmPlk4 CPBs revealed structural changes in the ZYG-1 CPB that confer selectivity for binding SPD-2 over Asterless-derived acidic regions. Overall, our findings suggest a conserved mechanism for centriolar docking of Plk4 homologs that initiate daughter centriole assembly. |
| Databáze: | OpenAIRE |
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