Up-regulation of long noncoding RNA CCAL predicts poor patient prognosis and promotes tumor metastasis in osteosarcoma
| Autor: | Xi-Wang Yang, Yongbo Yang, Na Wu, Da-Kai Zhou, Zhen-Jun Zhu, Huining Li |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Male Cancer Research Colorectal cancer Clinical Biochemistry Apoptosis Bone Neoplasms Biology Bioinformatics Real-Time Polymerase Chain Reaction Pathology and Forensic Medicine Metastasis 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Cell Movement medicine Biomarkers Tumor Tumor Cells Cultured Humans RNA Messenger Survival rate Cell Proliferation Neoplasm Staging Osteosarcoma Reverse Transcriptase Polymerase Chain Reaction RNA medicine.disease Prognosis Long non-coding RNA Survival Rate 030104 developmental biology Oncology Tumor progression 030220 oncology & carcinogenesis Cancer research Female RNA Long Noncoding Colorectal Neoplasms |
| Zdroj: | The International journal of biological markers. 32(1) |
| ISSN: | 1724-6008 |
| Popis: | Background Long noncoding RNAs (IncRNAs) play essential roles in tumor progression. Aberrant colorectal cancer-associated IncRNA (CCAL) has been found in colorectal cancer. However, the function of IncRNA CCAL in osteosarcoma (OS) remains unclear. Methods Quantitative real-time PCR (qRT-PCR) was performed to measure CCAL expression in OS tissues and adjacent nontumor tissues. The correlation betweent CCAL expression and clinicopathological features and prognosis was also analyzed. In addition, the function of CCAL was further evaluated by cell proliferation, migration and invasion assays. Results We showed that CCAL was significantly up-regulated in OS tissues compared with adjacent nontumor tissues. Increased expression of CCAL was correlated with advanced TNM stage and metastasis. Kaplan-Meier analysis demonstrated that patients with high CCAL expression had lower overall survival than those with low CCAL expression. Multivariate Cox regression analysis indicated that CCAL expression might be an independent prognostic factor for OS patients. In addition, functional assays showed that decreased CCAL expression could inhibit OS cell proliferation, migration and invasion ability. Conclusions Our findings suggested that CCAL plays critical roles in OS progression and could act as a therapeutic target in the treatment of OS. |
| Databáze: | OpenAIRE |
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