Live-cell imaging of glucose-induced metabolic coupling of β and α cell metabolism in health and type 2 diabetes
| Autor: | Peiyu Wang, Aleksey V. Matveyenko, Peter C. Butler, Scott E. Fraser, Madeline Rosenberger, Jason A. Junge, David Elashoff, Raymond C. Stevens, Tatyana Gurlo, Zhongying Wang, Kate L. White |
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| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine Fluorescence-lifetime imaging microscopy QH301-705.5 Medicine (miscellaneous) 030209 endocrinology & metabolism Oxidative phosphorylation Type 2 diabetes Carbohydrate metabolism Article Oxidative Phosphorylation General Biochemistry Genetics and Molecular Biology Diabetes Mellitus Experimental Rats Sprague-Dawley Mice 03 medical and health sciences Endocrinology 0302 clinical medicine Live cell imaging Insulin-Secreting Cells Diabetes mellitus medicine Animals Humans Glycolysis Biology (General) Chemistry Metabolism medicine.disease Islet Amyloid Polypeptide Molecular Imaging Rats Cell biology Glucose 030104 developmental biology Diabetes Mellitus Type 2 Microscopy Fluorescence Glucagon-Secreting Cells Rats Transgenic General Agricultural and Biological Sciences |
| Zdroj: | Communications Biology Communications Biology, Vol 4, Iss 1, Pp 1-11 (2021) |
| ISSN: | 2399-3642 |
| Popis: | Type 2 diabetes is characterized by β and α cell dysfunction. We used phasor-FLIM (Fluorescence Lifetime Imaging Microscopy) to monitor oxidative phosphorylation and glycolysis in living islet cells before and after glucose stimulation. In healthy cells, glucose enhanced oxidative phosphorylation in β cells and suppressed oxidative phosphorylation in α cells. In Type 2 diabetes, glucose increased glycolysis in β cells, and only partially suppressed oxidative phosphorylation in α cells. FLIM uncovers key perturbations in glucose induced metabolism in living islet cells and provides a sensitive tool for drug discovery in diabetes. Using phasor-FLIM (Fluorescence Lifetime Imaging Microscopy), Wang et al. reveal differential usages of oxidative phosphorylation and glycolysis in living islet cells following glucose stimulation. This study suggests the applicability of FLIM as a drug discovery tool as it reports perturbations in glucose metabolism in living islet cells. |
| Databáze: | OpenAIRE |
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