Changes in Actin Organization During the Cytotoxic Process
| Autor: | Radosevic, K., Radosevic, Katarina, van Leeuwen, Anne Marie T., Segers-Nolten, Gezina M.J., Figdor, Carl, de Grooth, B.G., Greve, Jan |
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| Přispěvatelé: | Faculty of Science and Technology, Nanobiophysics |
| Rok vydání: | 1994 |
| Předmět: |
Cytotoxicity
Immunologic Confocal Microscopy Natural killer Cell Biophysics macromolecular substances Biology Microfilament Filamentous actin Pathology and Forensic Medicine law.invention Mice F-actin Endocrinology Confocal microscopy law Quantification medicine Tumor Cells Cultured Cytotoxic T cell Animals Process (anatomy) Actin target cell Lasers Cell Biology Hematology Actins Cell biology Neoplasm Proteins Killer Cells Natural Actin Cytoskeleton medicine.anatomical_structure Microscopy Fluorescence IR-60719 Leukemia Erythroblastic Acute METIS-129464 K562 cells |
| Zdroj: | Cytometry, 15(4), 320-326. Wiley |
| ISSN: | 0196-4763 |
| DOI: | 10.1002/cyto.990150407 |
| Popis: | Changes in organization of F-actin during the cytotoxic process between NK and K562 cells have been observed and studied using confpcal laser scanning microscopy and quantitative fluorescence microscopy. An increase in F-actin content and orientation of F-actin towards the target cell have been observed in, conjugated NK cells. The increase in F-actin content probably reflects activation of the NK cell for the killing process. An increase in F-actin content in the conjugated K562 cell, occurring simultaneously with the appearance of filamentous actin structures that often originated/ended at the contact place with the NK cell, was also observed. These changes were delayed compared to the increase in F-actin content in the NK cell and were accompanied by increasing cytotoxic activity. This indicates that they were results of the interaction of the K562 cell with the activated NK cell. The possible role of target cell microfilaments in the cytotoxic process is addressed. |
| Databáze: | OpenAIRE |
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