Hepatic Nuclear Factor 1 (HNF1) shows a wider distribution than products of its known target genes in developing mouse
| Autor: | Hubert Condamine, M. Blumenfeld, Martine Maury, T. Chouard, Moshe Yaniv |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
medicine.medical_specialty
Transcription Genetic TATA box Gene Expression Mice Inbred Strains In situ hybridization Biology Kidney Mice Western blot Albumins Internal medicine medicine Animals Hepatocyte Nuclear Factor 1-alpha RNA Messenger Yolk sac Molecular Biology Gene Hepatocyte Nuclear Factor 1-beta Messenger RNA medicine.diagnostic_test Embryogenesis Genes Homeobox Nuclear Proteins Nucleic Acid Hybridization Embryo Mammalian Molecular biology DNA-Binding Proteins Antisense Elements (Genetics) Endocrinology medicine.anatomical_structure Liver Hepatocyte Nuclear Factor 1 Hepatocyte Nuclear Factor 1-Beta Transcription Factors Developmental Biology |
| Zdroj: | Development. 113:589-599 |
| ISSN: | 1477-9129 0950-1991 |
| Popis: | Hepatic nuclear factor 1 (HNF1) is a highly diverged homeoprotein that is crucial for transcription of many liver-specific genes including albumin. In particular, a minimal promoter, consisting of an HNF1-binding-site and a TATA box, is highly active only in hepatoma cell lines. The expression of the HNF1 and albumin genes has been examined in mouse embryos by in situ hybridization. At 10.5 days of gestation, the HNF1 mRNA was detected in both the hepatic primordia and visceral endoderm of the yolk sac whereas the albumin transcript was present only in the nascent liver. At later stages of development, HNF1 was detected in liver, in the epithelial cells of most of the digestive tract and in the cortex of the kidney, whereas albumin was again found only in the liver. The presence of HNF1 protein in adult kidney was demonstrated by immunodetection in gelretardation assays and western blot analysis. These experiments show that, even though the HNF1 homeoprotein is essential for expression of many liver-specific genes, it cannot, by itself, force high expression levels of these genes, in non-hepatic tissues. |
| Databáze: | OpenAIRE |
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