DMY is a Y-specific DM-domain gene required for male development in the medaka fish
| Autor: | Naoki Shibata, Nobuyoshi Shimizu, Craig E. Morrey, Hiroshi Hori, Masaru Matsuda, Satoshi Hamaguchi, Tadashi Sato, Shuichi Asakawa, Chika Matsuda, Tohru Kobayashi, Mitsuru Sakaizumi, Yoshitaka Nagahama, Ai Shinomiya |
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| Rok vydání: | 2002 |
| Předmět: |
Fish Proteins
Male X Chromosome Genotype Molecular Sequence Data Oryzias Biology Y chromosome Exon Animals Congenic Y Chromosome Animals Amino Acid Sequence RNA Messenger Cloning Molecular Gene Crosses Genetic In Situ Hybridization In Situ Hybridization Fluorescence Sequence Deletion Genetics Multidisciplinary Sexual differentiation Gene Expression Regulation Developmental Chromosome Breakage DM domain Sex Determination Processes XY sex-determination system Physical Chromosome Mapping Molecular biology Protein Structure Tertiary Phenotype Testis determining factor DMRT1 Gene Mutation Female human activities |
| Zdroj: | Nature. 417:559-563 |
| ISSN: | 1476-4687 0028-0836 |
| DOI: | 10.1038/nature751 |
| Popis: | Although the sex-determining gene Sry has been identified in mammals, no comparable genes have been found in non-mammalian vertebrates. Here, we used recombinant breakpoint analysis to restrict the sex-determining region in medaka fish (Oryzias latipes) to a 530-kilobase (kb) stretch of the Y chromosome. Deletion analysis of the Y chromosome of a congenic XY female further shortened the region to 250 kb. Shotgun sequencing of this region predicted 27 genes. Three of these genes were expressed during sexual differentiation. However, only the DM-related PG17 was Y specific; we thus named it DMY. Two naturally occurring mutations establish DMY's critical role in male development. The first heritable mutant--a single insertion in exon 3 and the subsequent truncation of DMY--resulted in all XY female offspring. Similarly, the second XY mutant female showed reduced DMY expression with a high proportion of XY female offspring. During normal development, DMY is expressed only in somatic cells of XY gonads. These findings strongly suggest that the sex-specific DMY is required for testicular development and is a prime candidate for the medaka sex-determining gene. |
| Databáze: | OpenAIRE |
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