Initiation of heteroduplex-loop repair by T4-encoded endonuclease VII in vitro
Autor: | Susanne Kleff, Börries Kemper |
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Rok vydání: | 1988 |
Předmět: |
Models
Molecular DNA Repair DNA repair Base pair Molecular Sequence Data Cleavage (embryo) General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Endonuclease Endodeoxyribonucleases Molecular Biology Binding Sites General Immunology and Microbiology biology Base Sequence General Neuroscience Nucleic Acid Heteroduplexes Molecular biology chemistry DNA Viral biology.protein Nucleic Acid Conformation T-Phages DNA Heteroduplex Research Article |
Zdroj: | The EMBO journal. 7(5) |
ISSN: | 0261-4189 |
Popis: | Heteroduplex DNAs with single-stranded loops of 51 nt or 8 nt were constructed in vitro and used in reactions with purified endonuclease VII (endo VII) from phage T4. The enzyme makes double-strand breaks by introducing pairs of staggered nicks flanking the loops. Regardless of loop-size the nicking sites map exclusively at the 3' side of the loop in the looping strand and at the 3' side of the base of the loop in the non-looping strand. The number of potential cleavage sites is small (less than 5) and their distribution depends on DNA sequence. The two closest staggered nicks are 4 bp apart, 2 bp on either side of the loop. Nicking always occurs in the double-stranded part of the molecules; the single-stranded loops are not attacked by endo VII. The nicks are introduced in a stepwise fashion and selection of the strand for the first nick depends on the sequence of 31 base pairs flanking the loops. |
Databáze: | OpenAIRE |
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