A New Fluorescence Detection Method for Tryptophan- and Tyrosine-Derived Allelopathic Compounds in Barley and Lupin

Autor: Sara Leite Dias, Adriana Garibay-Hernández, Fabian Leon Brendel, Benjamin Gabriel Chavez, Elena Brückner, Hans-Peter Mock, Jakob Franke, John Charles D’Auria
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Plants; Volume 12; Issue 10; Pages: 1930
ISSN: 2223-7747
DOI: 10.3390/plants12101930
Popis: Barley (Hordeum vulgare) is one of the most widely cultivated crops for feedstock and beer production, whereas lupins (Lupinus spp.) are grown as fodder and their seeds are a source of protein. Both species produce the allelopathic alkaloids gramine and hordenine. These plant-specialized metabolites may be of economic interest for crop protection, depending on their tissue distribution. However, in high concentrations they pose a health risk to humans and animals that feed on them. This study was carried out to develop and validate a new method for monitoring these alkaloids and their related metabolites using fluorescence detection. Separation was performed on an HSS T3 column using slightly acidified water-acetonitrile eluents. Calibration plots expressed linearity over the range 0.09–100 pmol/µL for gramine. The accuracy and precision ranged from 97.8 to 123.4%
Databáze: OpenAIRE