Generating an MEIS1 homozygous knockout human embryonic stem cell line using the CRISPR/Cas9 system
| Autor: | Yankun Yu, Xihong Lan, Li Wang, Fuxi Li, Canwei Zhang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Human Embryonic Stem Cells Biology Cell Line 03 medical and health sciences 0302 clinical medicine CRISPR Humans Clustered Regularly Interspaced Short Palindromic Repeats lcsh:QH301-705.5 Gene Embryonic Stem Cells Cas9 Embryogenesis Cell Biology General Medicine Embryonic stem cell Cell biology 030104 developmental biology lcsh:Biology (General) Cell culture CRISPR-Cas Systems 030217 neurology & neurosurgery Function (biology) Developmental Biology Human embryonic stem cell line |
| Zdroj: | Stem Cell Research, Vol 49, Iss, Pp 102069-(2020) |
| ISSN: | 1876-7753 |
| Popis: | Myeloid ecotropic viral integration site 1 (MEIS1) plays an essential role in the development of several embryonic organs, such as the central nervous system and eyes. To further investigate the role of MEIS1 in embryonic development, herein, we generated a MEIS1 homozygous knockout human embryonic stem cell (hESC) line using the CRISPR/Cas9 genome-editing technology. We believe that this cell line will be a good resource for exploring the function of the MEIS1 gene in embryonic development in vitro. Furthermore, the gene-knockout method reported in this study is efficient and labor-saving, which may provide an effective strategy for hESC gene deletion. |
| Databáze: | OpenAIRE |
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