Electrografted Poly(N-mercaptoethyl acrylamide) and Au Nanoparticles-Based Organic/Inorganic Film: A Platform for the High-Performance Electrochemical Biosensors
| Autor: | Yanfang Gao, Shoujiang Xu, Taihong Wang, Limiao Li, Jinghong Li, Zhifeng Du |
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| Rok vydání: | 2010 |
| Předmět: |
Polymers
Acrylic Resins Metal Nanoparticles Nanoparticle Nanotechnology Biosensing Techniques Electrochemistry Electrocatalyst Biochemistry Horseradish peroxidase Redox Catalysis chemistry.chemical_compound Electrodes Horseradish Peroxidase chemistry.chemical_classification biology Biomolecule Organic Chemistry Electrochemical Techniques General Chemistry Enzymes Immobilized chemistry Chemical engineering Acrylamide biology.protein Gold Oxidation-Reduction Biosensor |
| Zdroj: | Chemistry - An Asian Journal. 5:919-924 |
| ISSN: | 1861-471X 1861-4728 |
| DOI: | 10.1002/asia.200900490 |
| Popis: | In this study, we describe the use of the combination of eletrografting poly(N-mercaptoethyl acrylamide) and Au nanoparticles in the construction of high-performance biosensors. The poly(N-mercaptoethyl acrylamide) was electrografted onto the glassy carbon electrode surface, which provided a strongly adhering primer film for the stable attachment of Au nanoparticles and horseradish peroxidase (HRP) enzymes. The performances of the biosensors based on the HRP immobilized in the Au/poly(N-mercaptoethyl acrylamide) composite film were investigated. A couple of redox peaks were obtained, indicating that the Au nanoparticles could facilitate the direct-electron transfer between HRP and the underlying electrode. The biosensor showed an excellent electrocatalytic activity toward the reduction of hydrogen oxide and long-term stability, owing to the stable electrografted film and biocompatible Au nanoparticles. Our results demonstrate that the combination of electrografting and Au nanoparticles provides a promising platform for the immobilization of biomolecules and analysis of redox enzymes for their sensing applications. |
| Databáze: | OpenAIRE |
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