| Autor: |
Francesco Merola, Pier Luca Maffettone, Pietro Ferraro, Pasquale Memmolo, Erica Santonastaso, Massimiliano M. Villone, Gianluca Trotta |
| Přispěvatelé: |
Ferraro, Pietro, Villone, M. M., Santonastaso, E., Memmolo, P., Trotta, G., Merola, F., Maffettone, P. L., Ferraro, P. |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V. |
| DOI: |
10.1117/12.2592327 |
| Popis: |
The detection of CTCs in a blood sample is a challenging task due to their rarity and variety. We develop a new label-free and all-optical approach at the lab-on-chip scale for the detection of CTCs based on morphological biomarkers. In particular, we design a microfluidic device to be combined with a phase-contrast tomography system to carry out quantitative measurements of the three-dimensional structure of each single cell in a blood sample. In such device, two aspects are conjugated: on the one hand, the cells need to perform at least one complete rotation within the field of view of the imaging apparatus; on the other hand, the highest possible throughput has to be achieved, yet without deforming the cells significantly, which would impede their tomographic reconstruction. In this contribution, the finite-element-simulation-based preliminary design of a microfluidic device that would allow the achievement of the aforementioned objectives for cells with different shape and deformability is presented. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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