Mass spectrometric proteome analysis suggests anaerobic shift in metabolism of Dauer larvae of Caenorhabditis elegans
| Autor: | Hans Jürgen Thiesen, Bruno Ringel, András Mádi, Michael O. Glocker, Stefan Mikkat, Cornelia Koy |
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| Rok vydání: | 2008 |
| Předmět: |
Proteome
Hypothetical protein Molecular Sequence Data Biophysics Dauer larva Cell Fractionation Biochemistry Mass Spectrometry Analytical Chemistry Protein biosynthesis Animals Elméleti orvostudományok Amino Acid Sequence Anaerobiosis Caenorhabditis elegans Molecular Biology Alcohol dehydrogenase Inorganic pyrophosphatase Life Cycle Stages biology fungi Metabolism Orvostudományok biology.organism_classification Larva biology.protein |
| Zdroj: | Biochimica et biophysica acta. 1784(11) |
| ISSN: | 0006-3002 |
| Popis: | The Dauer larva is a non-feeding alternative larval stage of some nematodes specialized for long-term survival and dispersal. In this study we compared proteome maps obtained from Dauer larvae with those from the corresponding third larval stage (L3) of the feeding life cycle of C. elegans wild-type strain N2. We demonstrate at the protein level that altered metabolism may participate in longevity determination of Dauers. We detected huge amounts of alcohol dehydrogenase (CE12212) and aldehyde dehydrogenase (CE29809) in Dauer animals, indicating highly active fermentative pathways. Inorganic pyrophosphatase (CE05448) that enables to metabolize pyrophosphate as a high-energy source was over-expressed in Dauers. An interesting differentially expressed protein was phosphatidylethanolamine-binding protein (CE38516) that was found in high abundance in samples from Dauer larvae. Protein synthesis may be lowered in Dauer animals by the reduced expression of splicing factor rsp-3 (CE31089) and methionyl-tRNA synthase (CE34219). We observed significantly lower amounts of the pepsin-like aspartyl protease 1 (CE21681) in non-feeding Dauers, which is in agreement with reduced nutrient digestion. Finally, the hypothetical protein R08E5.2 (CE33294) was present in high abundance in L3 animals. |
| Databáze: | OpenAIRE |
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