Aluminium hydroxide down-regulates T helper 2 responses by allergen-stimulated human peripheral blood mononuclear cells
| Autor: | Louisa K. Wilcock, J.N. Francis, Stephen R. Durham |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Adult
Male T cell medicine.medical_treatment Immunology Antigen-Presenting Cells Down-Regulation Aluminum Hydroxide chemical and pharmacologic phenomena Biology complex mixtures Peripheral blood mononuclear cell Interferon-gamma chemistry.chemical_compound Th2 Cells Adjuvants Immunologic Antigens CD HLA Antigens medicine Humans Immunology and Allergy Interleukin 5 Cells Cultured Interleukin-13 Membrane Glycoproteins Alum Rhinitis Allergic Seasonal Allergens Interleukin-12 medicine.anatomical_structure chemistry Phleum Interleukin 13 B7-1 Antigen Leukocytes Mononuclear Interleukin 12 Pollen Female B7-2 Antigen Interleukin-4 Interleukin-5 Adjuvant CD80 |
| Zdroj: | Clinical Experimental Allergy. 34:1373-1378 |
| ISSN: | 1365-2222 0954-7894 |
| DOI: | 10.1111/j.1365-2222.2004.02052.x |
| Popis: | Summary Background Aluminium hydroxide (alum) is a commonly used adjuvant for specific immunotherapy of allergic diseases. While alum is traditionally associated with murine Th2 sensitization, little is known about its effects on secondary allergic responses in humans. Methods We investigated the in vitro effects of alum on peripheral blood mononuclear cells (PBMC) from atopic donors. PBMC from 18 grass pollen-sensitive rhinitic subjects were stimulated with Phleum pratense (Phl p) in the presence or absence of alum. After 6 days culture, cytokine production was measured by ELISA and T cell proliferation by radiolabelled thymidine incorporation. The effect of alum on the expression of human leucocyte antigen and CD80/CD86 on cultured antigen-presenting cells was assessed by flow cytometry. Results PBMC cultured with Phl p and alum showed a significant decrease in both IL-5 and IL-13 production compared with allergen alone (P |
| Databáze: | OpenAIRE |
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