Immunization with alkyl hydroperoxide reductase subunit C reduces Fusobacterium nucleatum load in the intestinal tract
| Autor: | Qiuyao Zeng, Songhe Guo, Ge Zhang, Zhi-Gang Nian, Yidan Wang, Hai-Fang Wang |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Science Reductase Article law.invention Microbiology Mice 03 medical and health sciences 0302 clinical medicine Immune system Bacterial Proteins Western blot law medicine Animals Humans Pathogen Mice Inbred BALB C Multidisciplinary biology medicine.diagnostic_test business.industry Peroxiredoxins Fusobacterium biology.organism_classification Antibodies Bacterial Bacterial Load Intestines 030104 developmental biology Immunization 030220 oncology & carcinogenesis Bacterial Vaccines Immunology Fusobacterium Infections biology.protein Recombinant DNA Medicine Female Antibody Fusobacterium nucleatum Colorectal Neoplasms business |
| Zdroj: | Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-10 (2017) |
| ISSN: | 2045-2322 |
| Popis: | Fusobacterium nucleatum (Fn) is an important tumour-associated bacterium in colorectal cancer (CRC). The antioxidant protein alkyl hydroperoxide reductase subunit C (AhpC) can induce strong antibacterial immune response during various pathogen infections. Our study aimed to evaluate the efficacy of Fn-AhpC as a candidate vaccine. In this work, by western blot analysis, we showed that Fn-AhpC recombinant protein could be recognized specifically by antibodies present in the sera of CRC patients; using the mouse Fn-infection model, we observed that systemic prophylactic immunization with AhpC/alum conferred significant protection against infection in 77.3% of mice. In addition, we measured the anti-AhpC antibody level in the sera of CRC patients and found that there was no obvious increase of anti-AhpC antibodies in the early-stage CRC group. Furthermore, we treated Fn with the sera from both immunized mice and CRC patients and found that sera with high anti-AhpC antibodies titre could inhibit Fn growth. In conclusion, our findings support the use of AhpC as a potential vaccine candidate against inhabitation or infection of Fn in the intestinal tract, which could provide a practical strategy for the prevention of CRC associated with Fn infection. |
| Databáze: | OpenAIRE |
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