Comprehensive Proteomic Analysis of the Human Amniotic Fluid Proteome: Gestational Age-Dependent Changes
| Autor: | Archana Thomas, Michael G. Gravett, Srinivasa R. Nagalla, Jodi Lapidus, Thomas Jacob, Ashok P. Reddy, Leonardo Pereira, John Edward A. Michaels, Surendra Dasari, Charles T. Roberts, Xinfang Lu, Matthew Rodland |
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| Rok vydání: | 2007 |
| Předmět: |
Proteomics
Serum Gel electrophoresis Amniotic fluid Proteome Age Factors Immunity Gestational age General Chemistry Fractionation Fetal health Biology Amniotic Fluid Biochemistry Molecular biology Transport protein Protein Transport Electrophoresis Pregnancy Humans Electrophoresis Gel Two-Dimensional Female Chromatography Liquid Signal Transduction |
| Zdroj: | Journal of Proteome Research. 6:1277-1285 |
| ISSN: | 1535-3907 1535-3893 |
| Popis: | Amniotic fluid (AF) is a significant contributor to fetal health and constitutes a potential rich source of biomarkers for diagnosis of maternal and fetal disorders. In this study, we performed a comprehensive survey of the proteins expressed in AF, combining gel and liquid-based fractionation approaches coupled with LC-MS/MS analysis. Two-dimensional Liquid Chromatography (2D-LC) analysis identified 118 nonredundant proteins with high confidence. One- and two-dimensional gel electrophoresis and in-gel digestion identified 101 proteins. Combining both sets resulted in 219 proteins, of which 96 are unique to AF; 70, 18, and 35 proteins are present in serum, cervico-vaginal fluid, and all three fluids, respectively. Fluorescence two-dimensional differential in-gel electrophoresis (2D-DIGE) comparison of first-, second-, and third-trimester AF samples revealed that maximal differences in the relative abundance of AF proteins occur between the first and second trimesters. A systematic analysis of proteins present both in AF and maternal serum could lead to the development of new noninvasive diagnostic procedures to monitor fetal status. |
| Databáze: | OpenAIRE |
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