Absence of delayed chromosomal instability in a normal human fibroblast cell line after 125 I iododeoxyuridine

Autor: R. J. Mairs, A. Neshasateh-Riz, T. E. Wheldon, C. S. Griffin, E. G. Wright
Rok vydání: 2000
Předmět:
Zdroj: International Journal of Radiation Biology. 76:963-969
ISSN: 1362-3095
0955-3002
DOI: 10.1080/09553000050050972
Popis: Purpose : To seek the delayed appearance of chromosomal abnormalities in human fibroblasts exposed to the Auger electron emitter 125 I. Materials and methods : Normal untransformed human fibroblasts, HF19, were exposed to a concentration of [I 125 ]IUdR, which allowed the survival of 37% of clonogens. Chromosomal analysis using both conventional Giemsa and fluorescence in situ hybridization (FISH) was undertaken on non-clonal bulk cultures from 2 to 39 days after treatment. Results : The data show a declining level of unstable aberrations in the progeny of HF19 fibroblasts exposed to [I 125 ]IUdR, eventually reaching control levels. Conclusions : The results provide evidence that [ 125 I]IUdR does not induce ongoing chromosomal instability in long-term culture, and gives further support to the use of Auger-electron emitting radionuclides in the treatment and diagnosis of tumours.
Databáze: OpenAIRE