Absence of delayed chromosomal instability in a normal human fibroblast cell line after 125 I iododeoxyuridine
Autor: | R. J. Mairs, A. Neshasateh-Riz, T. E. Wheldon, C. S. Griffin, E. G. Wright |
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Rok vydání: | 2000 |
Předmět: |
Chromosome Aberrations
Genetics Radiological and Ultrasound Technology medicine.diagnostic_test Chromosomal analysis Fibroblasts Biology Fibroblast cell line Molecular biology Giemsa stain Cell Line Iodine Radioisotopes medicine.anatomical_structure Cell culture Idoxuridine Chromosome instability medicine Humans Radiology Nuclear Medicine and imaging Fibroblast In Situ Hybridization Fluorescence After treatment Fluorescence in situ hybridization |
Zdroj: | International Journal of Radiation Biology. 76:963-969 |
ISSN: | 1362-3095 0955-3002 |
DOI: | 10.1080/09553000050050972 |
Popis: | Purpose : To seek the delayed appearance of chromosomal abnormalities in human fibroblasts exposed to the Auger electron emitter 125 I. Materials and methods : Normal untransformed human fibroblasts, HF19, were exposed to a concentration of [I 125 ]IUdR, which allowed the survival of 37% of clonogens. Chromosomal analysis using both conventional Giemsa and fluorescence in situ hybridization (FISH) was undertaken on non-clonal bulk cultures from 2 to 39 days after treatment. Results : The data show a declining level of unstable aberrations in the progeny of HF19 fibroblasts exposed to [I 125 ]IUdR, eventually reaching control levels. Conclusions : The results provide evidence that [ 125 I]IUdR does not induce ongoing chromosomal instability in long-term culture, and gives further support to the use of Auger-electron emitting radionuclides in the treatment and diagnosis of tumours. |
Databáze: | OpenAIRE |
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