Structural Characterization of an Exopolysaccharide Isolated from Enterococcus faecalis, and Study on its Antioxidant Activity, and Cytotoxicity Against HeLa Cells
| Autor: | Gajendra Nath Maity, Soumitra Mondal, Bikas R. Pati, Kalyani Khanra, Indranil Choudhuri, Prasenjit Pariya, Nandan Bhattacharyya |
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| Rok vydání: | 2020 |
| Předmět: |
Antioxidant
medicine.medical_treatment Applied Microbiology and Biotechnology Microbiology Enterococcus faecalis Antioxidants HeLa 03 medical and health sciences chemistry.chemical_compound Hydrolysis medicine Monosaccharide Humans Cytotoxicity 030304 developmental biology chemistry.chemical_classification 0303 health sciences biology 030306 microbiology Polysaccharides Bacterial General Medicine biology.organism_classification Ascorbic acid HEK293 Cells chemistry Biochemistry Hydroxyl radical HeLa Cells |
| Zdroj: | Current microbiology. 77(10) |
| ISSN: | 1432-0991 |
| Popis: | An exopolysaccharide (EPS-I) having the molecular weight ~ 2.6 × 105 Da, was isolated from a Zinc resistant strain of Enterococcus faecalis from costal area. The exopolysaccharide consists of D-mannose, D-glucose, and L-fucose in molar ratio of 9:4:1. The monosaccharide units in the EPS-1 were determined through chemical (total acid hydrolysis and methylation analysis) and spectroscopic (FTIR and 1H NMR experiment) analysis. The mannose-rich EPS-1 showed total antioxidant activity (1 mg mL−1 of EPS-I as functional as approximately to 500 ± 5.2 µM of ascorbic acid) and Fe2+ metal ion chelation activity (EC50 = 405.6 µg mL−1) and hydroxyl radical scavenging activity (EC50 = 219.5 µg mL−1). The in vitro cytotoxicity experiment of EPS-I against cervical carcinoma cell line, HeLa cells showed strong cytotoxic effect (LC50 = 267.3 µg mL−1) and at that concentration, it found almost nontoxic against normal healthy cells (HEK-293). |
| Databáze: | OpenAIRE |
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