Endogenous and exogenous pituitary-specific promoters are differentially controlled
| Autor: | A.M. Rankin, D.R. McWilliams, P.J. Hippenmeyer, R.A. Wolfe, B.B. Brightwell, B.A. Reitz, T.G. Warren |
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| Rok vydání: | 1995 |
| Předmět: |
Transcriptional Activation
endocrine system Molecular Sequence Data Endogeny CHO Cells Biology Biochemistry Cell Line Transactivation Endocrinology Genes Reporter Cricetinae Protein biosynthesis Animals Promoter Regions Genetic Molecular Biology DNA Primers Reporter gene Base Sequence Chinese hamster ovary cell Gene Transfer Techniques Promoter Transfection Molecular biology Prolactin Rats DNA-Binding Proteins Growth Hormone Pituitary Gland Tissue Plasminogen Activator Genetic Engineering Transcription Factor Pit-1 Transcription Factors |
| Zdroj: | Molecular and cellular endocrinology. 107(2) |
| ISSN: | 0303-7207 |
| Popis: | We have engineered GH3 cells with reporter genes under control of the growth hormone and prolactin promoters and measured protein production. The results indicate very low level production of reporter proteins from the cells regardless of the promoter used to drive expression. This was surprising in light of the observation that the cells still produced high levels of endogenous growth hormone and prolactin. Chinese hamster ovary (CHO) cells were engineered to express the Pit-1 transactivator. Transfection of reporter genes under control of the prolactin promoter demonstrated a clear enhancement of expression levels compared to the same promoter in parental CHO cells. Pit-1 expression is not sufficient, however, for high level, stable expression from the growth hormone promoter. These results indicate that the growth hormone and prolactin promoters are not sufficient for high level, stable expression even in normally permissive cells and suggest that Pit-1 alone is not sufficient for strong promoter activity from the integrated plasmids. |
| Databáze: | OpenAIRE |
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