Nutrient depletion and TOR inhibition induce 18S and 25S ribosomal RNAs resistant to a 5′-phosphate-dependent exonuclease in Candida albicans and other yeasts
| Autor: | Jacob Fleischmann, Miguel A. Rocha |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Exonuclease lcsh:QH426-470 Physiological 030106 microbiology 18S Medical Biochemistry and Metabolomics Stress Ribosome 03 medical and health sciences Opthalmology and Optometry Stress Physiological Candida albicans Genetics RNA Ribosomal 18S Terminator lcsh:QH573-671 Pyrophosphatases Protein Kinase Inhibitors Molecular Biology Ribosomal Sirolimus Messenger RNA biology lcsh:Cytology TOR Serine-Threonine Kinases RNA RNA Fungal Ribosomal RNA Alkaline Phosphatase biology.organism_classification Yeast lcsh:Genetics Fungal 030104 developmental biology Terminator (genetics) Biochemistry Phosphodiesterase I RNA Ribosomal biology.protein Biochemistry and Cell Biology Developmental Biology Research Article |
| Zdroj: | BMC Molecular Biology BMC molecular biology, vol 19, iss 1 BMC Molecular Biology, Vol 19, Iss 1, Pp 1-8 (2018) |
| ISSN: | 1471-2199 |
| DOI: | 10.1186/s12867-018-0102-y |
| Popis: | Background Messenger RNA (mRNA) represents a small percentage of RNAs in a cell, with ribosomal RNA (rRNA) making up the bulk of it. To isolate mRNA from eukaryotes, typically poly-A selection is carried out. Recently, a 5´-phosphate-dependent, 5´→3´ processive exonuclease called Terminator has become available. It will digest only RNA that has a 5´-monophosphate end and therefore it is very useful to eliminate most of rRNAs in cell. Results We have found that in the pathogenic yeast Candida albicans, while 18S and 25S components isolated from yeast in robust growth phase are easily eliminated by Terminator, those isolated from cells in the nutritionally diminished stationary phase, become resistant to digestion by this enzyme. Additional digestions with alkaline phosphatase, tobacco pyrophosphatase combined with Terminator point toward the 5′-prime end of 18S and 25S as the source of this resistance. Inhibition of TOR by rapamycin also induces resistance by these molecules. We also find that these molecules are incorporated into the ribosome and are not just produced incidentally. Finally, we show that three other yeasts show the same behavior. Conclusions Digestion of RNA by Terminator has revealed 18S and 25S rRNA molecules different from the accepted processed ones seen in ribosome generation. The reason for these molecules and the underlying mechanism for their formation is unknown. The preservation of this behavior across these yeasts suggests a useful biological role for it, worthy of further inquiry. |
| Databáze: | OpenAIRE |
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