Modulation of the cellulose content of tuber cell walls by antisense expression of different potato (Solanum tuberosum L.) CesA clones

Autor: Henk A. Schols, Edwin J. Bakx, Richard G. F. Visser, Emmanouil N. Tzitzikas, Jean-Paul Vincken, Maxwell S. Bush, Ronald J.F.J. Oomen, Irma Straatman-Engelen, Maureen C. McCann
Jazyk: angličtina
Rok vydání: 2004
Předmět:
Starch
polysaccharides
Gene Expression
Plant Science
Biochemistry
chemistry.chemical_compound
Laboratorium voor Plantenveredeling
Cell Wall
catalytic subunit
Spectroscopy
Fourier Transform Infrared
Phylogeny
Plant Proteins
mutants
chemistry.chemical_classification
biology
Food Chemistry
Reverse Transcriptase Polymerase Chain Reaction
plants
EPS-3
Monosaccharides
acetobacter-xylinum
infrared-spectra
food and beverages
General Medicine
Plants
Genetically Modified
synthase gene-expression
Glucosyltransferases
Multigene Family
Colorimetry
Starch synthase
Solanaceae
DNA
Complementary
Horticulture
Polysaccharide
Cell wall
resistance
Transformation
Genetic
Complementary DNA
Levensmiddelenchemie
RNA
Antisense
Cellulose
Molecular Biology
Solanum tuberosum
VLAG
cDNA library
fungi
granule
biology.organism_classification
Plant Breeding
arabidopsis
chemistry
biology.protein
Zdroj: Phytochemistry, 65(5), 535-546
Phytochemistry 65 (2004) 5
ISSN: 0031-9422
DOI: 10.1016/j.phytochem.2003.12.019
Popis: Four potato cellulose synthase (CesA) homologs (StCesA1, 2, 3 and 4) were isolated by screening a cDNA library made from developing tubers. Based on sequence comparisons and the fact that all four potato cDNAs were isolated from this single cDNA-library, all four StCesA clones are likely to play a role in primary cell wall biosynthesis. Several constructs were generated to modulate cellulose levels in potato plants in which the granule-bound starch synthase promoter was used to target the modification to the tubers. The StCesA3 was used for up- and down-regulation of the cellulose levels by sense (SE-StCesA3) and antisense (AS-StCesA3) expression of the complete cDNA. Additionally, the class-specific regions (CSR) of all four potato cellulose synthase genes were used for specific down-regulation (antisense) of the corresponding CesA genes (csr1, 2, 3 and 4). None of the transformants showed an overt developmental phenotype. Sections of tubers were screened for altered cell wall structure by Fourier Transform Infrared microspectroscopy (FTIR) and exploratory Principal Component Analysis (PCA), and those plants discriminating from WT plants were analysed for cellulose content and monosaccharide composition. Several transgenic lines were obtained with mainly decreased levels of cellulose. These results show that the cellulose content in potato tubers can be reduced down to 40% of the WT level without affecting normal plant development, and that constructs based on the CSR alone are specific and sufficient to down-regulate cellulose biosynthesis. (C) 2004 Elsevier Ltd. All rights reserved.
Databáze: OpenAIRE
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