Aptamer-based biosensor for sensitive PDGF detection using diamond transistor
Autor: | Robert Edgington, A. Rahim Ruslinda, Yoko Ishii, Hiroshi Kawarada, Shinya Tajima, Yuichiro Ishiyama |
---|---|
Rok vydání: | 2010 |
Předmět: |
Transistors
Electronic Aptamer Becaplermin Biomedical Engineering Biophysics Nanotechnology Biosensing Techniques Electrolyte engineering.material Sensitivity and Specificity law.invention law Equipment Reuse Electrochemistry Platelet-Derived Growth Factor Base Sequence Chemistry Transistor Diamond Proto-Oncogene Proteins c-sis General Medicine Aptamers Nucleotide Covalent bond engineering Degradation (geology) Biosensor Layer (electronics) Biotechnology |
Zdroj: | Biosensors and Bioelectronics. 26:1599-1604 |
ISSN: | 0956-5663 |
Popis: | The detection of platelet-derived growth factor (PDGF) via a solution-gate field-effect transistor (SGFET) has been demonstrated for the first time using aptamers immobilized on a diamond surface. Upon introduction of PDGF to the immobilized aptamer, a shift of 31.7 mV in the negative direction is observed at a source-drain current of −50 μA. A shift of 32.3 mV in the positive direction is detected after regeneration by SDS solution, indicating that the static measurement returns to its original value. These SGFETs operate stably within the large potential window of diamond (>3.0 V), and hence the surface channel does not need passivating with a thick insulating layer. Thereof, the immobilized aptamer channels have been exposed directly to the electrolyte solution without a gate insulator. Immobilization is achieved via aptamers covalently bonding to amine sites, thereby increasing the sensitivity of the biosensors. Diamond SGFETs have potential for the detection of PDGF and show durability against biological degradation after repeated usage and regeneration. |
Databáze: | OpenAIRE |
Externí odkaz: |