Differential roles of orexin receptors within the dentate gyrus in stress- and drug priming-induced reinstatement of conditioned place preference in rats
| Autor: | Fereshte Ebrahimian, Farzaneh Sadat Naghavi, Zahra Taslimi, Fatemeh Yazdi, Fatemeh Sadeghzadeh, Abbas Haghparast |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Male Time Factors medicine.drug_class Pyridines Drug-Seeking Behavior Stimulation Pharmacology Extinction Psychological 03 medical and health sciences Behavioral Neuroscience 0302 clinical medicine Reward Orexin Receptors Conditioning Psychological medicine Animals Urea Naphthyridines Rats Wistar Receptor Swimming Benzoxazoles Dose-Response Relationship Drug Morphine Dentate gyrus TCS-OX2-29 Receptor antagonist Isoquinolines Orexin receptor Conditioned place preference Rats Analgesics Opioid 030104 developmental biology Dentate Gyrus Conditioning Operant Orexin Receptor Antagonists Psychology Neuroscience 030217 neurology & neurosurgery Stress Psychological medicine.drug |
| Zdroj: | Behavioral neuroscience. 130(1) |
| ISSN: | 1939-0084 |
| Popis: | Orexins are hypothalamic peptides involved in the modulation of the feeding, arousal, reward function, learning, and memory; nevertheless, the role of orexins in stress and relapse are largely unclear. Therefore, in the present study, the reinstatement model were used to examine the effects of intradentate gyrus (DG) administration of SB334867 as an orexin-1 receptor antagonist and TCS OX2 29, as an orexin-2 receptor antagonist on drug priming- and forced swim stress (FSS)-induced reinstatement of morphine. One-hundred and 44 adult male albino Wistar rats weighing 200 g-280 g were bilaterally implanted by cannulas into the DG. For induction of conditioned place preference (CPP), subcutaneous (sc) injection of morphine (5 mg/kg) was used daily during a 3-day conditioning phase. Then, the conditioning score (conditional stimulus [CS]) was calculated. After a 24 hr "off" period following achievement of extinction criterion, rats were tested for drug priming-induced reinstatement by priming dose of morphine (1 mg/kg, sc) and for FSS-induced reinstatement 10 min after FSS. In the next experiments, animals received different doses of intra-DG administration of SB334867 and TCS OX2 29 (3, 10, and 30 μg/0.5 μl 12% DMSO per side), bilaterally and were subsequently tested for morphine priming- and FSS-induced reinstatement. Our findings indicated that the FSS-induced the reinstatement of seeking behaviors. Furthermore, intra-DG administration of orexin-1 and orexin-2 receptor antagonists attenuated drug priming-induced reinstatement dose-dependently. However, they have trivial role in FSS-induced reinstatement. It is concluded that drug priming-induced reinstatement may be mediated, at least in part, by stimulation of orexin receptors in the DG. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|