Ancillary p16(INK4a) adds no meaningful value to the performance of ER/PR/Vim/CEA panel in distinguishing between primary endocervical and endometrial adenocarcinomas in a tissue microarray study
| Autor: | Ya Wen Cheng, Ming-Yung Lee, Yeu Sheng Tyan, Mei Fen Kung, Chih Ping Han, Tina S. Wu, Po Hui Wang, Chung Chin Yao, Lai Fong Kok |
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| Rok vydání: | 2008 |
| Předmět: |
Pathology
medicine.medical_specialty Scoring system H&E stain Protein Array Analysis Value (computer science) Uterine Cervical Neoplasms Vimentin Adenocarcinoma Carcinoembryonic antigen Progesterone receptor medicine Biomarkers Tumor Humans neoplasms Cyclin-Dependent Kinase Inhibitor p16 Tissue microarray biology business.industry Obstetrics and Gynecology General Medicine Immunohistochemistry Carcinoembryonic Antigen Endometrial Neoplasms Receptors Estrogen biology.protein Female business Receptors Progesterone |
| Zdroj: | Archives of gynecology and obstetrics. 280(3) |
| ISSN: | 1432-0711 |
| Popis: | Endocervical adenocarcinomas (ECA) and endometrial adenocarcinomas (EMA) are uterine malignancies that have differing biological behavior. The choice of appropriate therapeutic plan depends indeed on the tumor’s site of origin. In this study, we not only compare the individual expression status of five immunomarkers (ER, PR, Vim, CEA, and p16INK4a), but also evaluate whether p16INK4a adds value to the ER/PR/Vim/CEA panel characteristics in distinguishing between primary ECA and EMA. A tissue microarray (TMA) was constructed using paraffin-embedded, formalin-fixed tissues from 35 hysterectomy specimens, including 14 ECA and 21 EMA. TMA sections were immunostained with five anti-bodies, by avidin–biotin complex (ABC) method for antigen visualization. The staining intensity and area extent of the immunohistochemical (IHC) reactions were appraised by using the semi-quantitative scoring system. The four respective markers (ER, PR, Vim, CEA) and their combined panel expressions showed significant (p |
| Databáze: | OpenAIRE |
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