Value of Routine Dengue Diagnostic Tests in Urine and Saliva Specimens
| Autor: | Anavaj Sakuntabhai, Anne-Claire Andries, Patrich Lorn Try, Sopheaktra Ros, Kim Srorn Kim, Paul F. Horwood, Julien Cappelle, Marie Flamand, Sowath Ly, Veasna Duong, Arnaud Tarantola, Philippe Buchy, Sivuth Ong, Rekol Huy |
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| Přispěvatelé: | Institut Pasteur du Cambodge, Réseau International des Instituts Pasteur (RIIP), Animal et gestion intégrée des risques (UPR AGIRs), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Pediatric Department [Cambodia], Kampong Cham Provincial hospital [Cambodia], Centre National de Malariologie (CNM), Virologie Structurale - Structural Virology, Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Génétique fonctionnelle des Maladies infectieuses - Functional Genetics of Infectious Diseases, GlaxoSmithKline Vaccines [Singapore], GlaxoSmithKline [Headquarters, London, UK] (GSK), The research leading to these results has received funding from the European Union Seventh Framework Programme (FP7/2007/2011) under Grant Agreement n°282378., European Project: 282378,EC:FP7:HEALTH,FP7-HEALTH-2011-single-stage,DENFREE(2012), Institut Pasteur du Cambodge-Réseau International des Instituts Pasteur ( RIIP ), Animal et gestion intégrée des risques ( Agirs ), Centre de Coopération Internationale en Recherche Agronomique pour le Développement ( CIRAD ), Kampong Cham Provincial hospital, National Center of Parasitology ( CNM ), National Malaria Center [Phnom Penh], Département de Virologie, Institut Pasteur [Paris], Génétique fonctionnelle des Maladies infectieuses, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS ), The research leading to these results has received funding from the European Union Seventh Framework Programme (FP7/2007/2011) under Grant Agreement n°282 378., European Project : 282378,EC:FP7:HEALTH,FP7-HEALTH-2011-single-stage,DENFREE ( 2012 ), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
MESH: Viral Nonstructural Proteins/urine Urine Dengue virus Antibodies Viral MESH: Immunoglobulin Isotypes/analysis Santé publique Polymerase Chain Reaction Gastroenterology MESH: Antibodies Viral/blood Plasma MESH : Child MESH: Child MESH : Dengue Virus Child MESH : Viral Nonstructural Proteins MESH: Dengue Virus/isolation & purification MESH : Diagnostic Tests Routine Blood proteins MESH : Enzyme-Linked Immunosorbent Assay 3. Good health Immunoglobulin Isotypes [ SDV.MHEP.MI ] Life Sciences [q-bio]/Human health and pathology/Infectious diseases MESH : Urine [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Child Preschool Disease Progression MESH: Disease Progression MESH: Dengue/urine Antibody Genre humain medicine.medical_specialty lcsh:RC955-962 MESH: Dengue/epidemiology MESH: Saliva/virology MESH: Urine/chemistry MESH: Plasma/immunology MESH: RNA Viral/isolation & purification MESH : Adolescent Humans Epidemics Saliva Technique analytique MESH: Diagnostic Tests Routine Test biologique MESH: Adolescent MESH: Humans MESH: Viral Nonstructural Proteins/blood [ SDV ] Life Sciences [q-bio] MESH: Dengue/blood Diagnostic Tests Routine MESH : Cambodia MESH : Humans MESH: Child Preschool Public Health Environmental and Occupational Health lcsh:RA1-1270 MESH: Polymerase Chain Reaction MESH : Disease Progression Dengue Virus medicine.disease Immunology MESH: Antibodies Viral/urine MESH : Saliva MESH: Female MESH: Dengue Virus/immunology Sang Viral Nonstructural Proteins MESH : Child Preschool medicine.disease_cause MESH: Immunoglobulin Isotypes/blood Serology Dengue fever Dengue Blood plasma MESH : Female MESH : Polymerase Chain Reaction Salive MESH: Dengue/diagnosis MESH : Epidemics lcsh:Public aspects of medicine MESH: Enzyme-Linked Immunosorbent Assay MESH : Immunoglobulin Isotypes MESH: Viral Nonstructural Proteins/analysis MESH: Immunoglobulin Isotypes/urine Infectious Diseases S50 - Santé humaine MESH: Urine/virology RNA Viral Female MESH: Genome Viral Cambodia MESH: Dengue Virus/genetics MESH : Genome Viral Research Article MESH : Antibodies Viral MESH: Saliva/immunology lcsh:Arctic medicine. Tropical medicine Adolescent MESH : Male MESH : Dengue Enzyme-Linked Immunosorbent Assay Genome Viral Biology Diagnostic de laboratoire MESH: Antibodies Viral/analysis Internal medicine medicine MESH : RNA Viral MESH: Epidemics MESH: Plasma/chemistry MESH : Plasma MESH: Urine/physiology Flavivirus MESH: Cambodia MESH: Male biology.protein MESH: Saliva/chemistry U30 - Méthodes de recherche MESH: Plasma/virology |
| Zdroj: | PLoS Neglected Tropical Diseases PLoS Neglected Tropical Diseases, Public Library of Science, 2015, 9 (9), pp.e0004100. ⟨10.1371/journal.pntd.0004100⟩ PLoS Neglected Tropical Diseases, Vol 9, Iss 9, p e0004100 (2015) PLoS Neglected Tropical Diseases, Public Library of Science, 2015, 9 (9), pp.e0004100. 〈10.1371/journal.pntd.0004100〉 PLoS Neglected Tropical Diseases, 2015, 9 (9), pp.e0004100. ⟨10.1371/journal.pntd.0004100⟩ |
| ISSN: | 1935-2727 1935-2735 |
| DOI: | 10.1371/journal.pntd.0004100⟩ |
| Popis: | Background Dengue laboratory diagnosis is essentially based on detection of the virus, its components or antibodies directed against the virus in blood samples. Blood, however, may be difficult to draw in some patients, especially in children, and sampling during outbreak investigations or epidemiological studies may face logistical challenges or limited compliance to invasive procedures from subjects. The aim of this study was to assess the possibility of using saliva and urine samples instead of blood for dengue diagnosis. Methodology/Principal Findings Serial plasma, urine and saliva samples were collected at several time-points between the day of admission to hospital until three months after the onset of fever in children with confirmed dengue disease. Quantitative RT-PCR, NS1 antigen capture and ELISA serology for anti-DENV antibody (IgG, IgM and IgA) detection were performed in parallel on the three body fluids. RT-PCR and NS1 tests demonstrated an overall sensitivity of 85.4%/63.4%, 41.6%/14.5% and 39%/28.3%, in plasma, urine and saliva specimens, respectively. When urine and saliva samples were collected at the same time-points and tested concurrently, the diagnostic sensitivity of RNA and NS1 detection assays was 69.1% and 34.4%, respectively. IgG/IgA detection assays had an overall sensitivity of 54.4%/37.4%, 38.5%/26.8% and 52.9%/28.6% in plasma, urine and saliva specimens, respectively. IgM were detected in 38.1% and 36% of the plasma and saliva samples but never in urine. Conclusions Although the performances of the different diagnostic methods were not as good in saliva and urine as in plasma specimens, the results obtained by qRT-PCR and by anti-DENV antibody ELISA could well justify the use of these two body fluids to detect dengue infection in situations when the collection of blood specimens is not possible. Author Summary Dengue is the most important arthropod-borne disease affecting humans and represents a huge public health burden in affected countries. Symptoms are often non-specific hence the need for an early, sensitive and specific diagnosis of dengue for appropriate management as well as for early epidemic detection. Currently, almost all laboratory diagnostic methods require a blood specimen that may be sometimes be difficult or inconvenient to obtain. In this study, we assessed the possibility to use saliva and urine samples as alternatives to blood specimens in dengue diagnosis. We demonstrated that the performances of the different diagnostic methods (RT-PCR, NS1 antigen detection and anti-DENV IgM/IgG/IgA ELISAs) were in general not as good in saliva and urine as in plasma, but that the use of these body fluids obtained by non-invasive methods could be of value in certain circumstances such as outbreak investigations or in young children (once they are old enough to comply to instructions), in addition to the situations when blood cannot be easily collected (e.g., lack of phlebotomist, refusal of the procedure, etc.). |
| Databáze: | OpenAIRE |
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