Separation of the Glycosylated Carotenoid Myxoxanthophyll from Synechocystis Salina by HPCCC and Evaluation of Its Antioxidant, Tyrosinase Inhibitory and Immune-Stimulating Properties
| Autor: | José Cheel, Tereza Fábryová, Michaela Nováková, Doris Vokurková, Jiří Kopecký, Lenka Tůmová, Iva Dolečková, Pavel Hrouzek |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Cyanobacteria Antioxidant Tyrosinase medicine.medical_treatment centrifugal partition chromatography (CPC) countercurrent chromatography (CCC) Filtration and Separation Synechocystis cyanobacteria 01 natural sciences High-performance liquid chromatography Analytical Chemistry lcsh:Chemistry Pigment Countercurrent chromatography medicine Carotenoid chemistry.chemical_classification Chromatography biology 010604 marine biology & hydrobiology 010401 analytical chemistry biology.organism_classification lcsh:QC1-999 0104 chemical sciences high-performance countercurrent chromatography (HPCCC) lcsh:QD1-999 chemistry visual_art visual_art.visual_art_medium bacteria myxoxanthophyll lcsh:Physics |
| Zdroj: | Separations Volume 7 Issue 4 Separations, Vol 7, Iss 73, p 73 (2020) |
| ISSN: | 2297-8739 |
| DOI: | 10.3390/separations7040073 |
| Popis: | Global demand for natural pigments has increased in the past few years. Myxoxanthophyll, a glycosylated monocyclic carotenoid, is a pigment that occurs naturally in cyanobacteria but no scalable isolation process has been developed to obtain it from its natural source to date. In this study, myxoxanthophyll was isolated from unicellular cyanobacterium Synechocystis salina (S. salina) using high-performance countercurrent chromatography (HPCCC), where the lower phase of the biphasic solvent system composed of n-heptane&ndash ethanol&ndash water (2:4:4, v/v/v) was used as a mobile phase, whereas its upper phase was the stationary phase. For the HPCCC isolation, a multi-injection method was developed, and four consecutive sample injections (70 mg each) were performed, obtaining, in total, 20 mg of myxoxanthophyll, which was finally purified with high-performance liquid chromatography (HPLC). Overall, a final myxoxanthophyll yield of 15 mg (98% purity) was obtained. The target pigment showed a weak antioxidant and tyrosinase inhibitory effect, and exhibited immune-stimulating properties by activating human granulocytes. The results presented here form a basis for the large-scale production of myxoxanthophyll, and show the potential benefits of this pigment for human health. |
| Databáze: | OpenAIRE |
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